Contractile response of lipopolysaccharide-treated rat thoracic aortas to adrenaline or noradrenaline with or without local anaesthetic agents

Aim: In our previous study, we demonstrated that the vasoconstrictive action of lidocaine occurs at a concentration of 10^-4 mol/L. Furthermore, we found that lidocaine enhanced contractile responses produced by low concentrations of adrenaline (10^-8-10^-7 mol/L). In this study, the responses of isolated rat thoracic aorta preparations to local anaesthetic solutions containing either prilocaine or mepivacaine were examined in the presence of adrenaline or noradrenaline. Methods: We isolated thoracic aorta from Wister rats. The arteries were cut into ~3 mm long rings that were stretched by a pair of hooks in an organ bath filled with Krebs-Henseleit solution. After 1 µg/ml lipopolysaccharide treatment, vasoconstrictors (either adrenaline or noradrenaline) were applied in a cumulative manner between concentrations of 10-9–10-5 mol/L of either prilocaine or mepivacaine (10^-4 mol/L). Changes in isometric vasocontractions were continuously recorded with an amplifier system using the PCD-30A computer system. Results: The contractile response to both prilocaine and mepivacaine significantly decreased at low concentrations of adrenaline (10^-8-10^-7 mol/L). The contractile response to mepivacaine also significantly decreased at low concentrations of adrenaline (10^-9-10^-8 mol/L). In contrast, with the exception of prilocaine at a low concentration of adrenaline (10^-8 mol/L), neither prilocaine nor mepivacaine significantly decreased the contractile responses. In a model of blood vessel inflammation under 6 h lipopolysaccharide treatment, prilocaine and mepivacaine enhanced contractile responses produced by 10^-9-10^-7 mol/L noradrenaline. Conclusion: Noradrenaline should be administered when injecting inflamed tissues with prilocaine or mepivacaine.