Contractile response of lipopolysaccharide-treated rat thoracic aortas to adrenaline or noradrenaline with or without local anaesthetic agents

Shigeki Joseph Luke Fujiwara, Michiko Yasuda, Tomoko Kinugawa, Shoko Itakura, Kazu Ichi Yoshida, Hiroshi Kitahata, Takeshi Yokoyama

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: In our previous study, we demonstrated that the vasoconstrictive action of lidocaine occurs at a concentration of 10-4 mol/L. Furthermore, we found that lidocaine enhanced contractile responses produced by low concentrations of adrenaline (10-8-10-7 mol/L). In this study, the responses of isolated rat thoracic aorta preparations to local anaesthetic solutions containing either prilocaine or mepivacaine were examined in the presence of adrenaline or noradrenaline. Methods: We isolated thoracic aorta from Wister rats. The arteries were cut into ~3 mm long rings that were stretched by a pair of hooks in an organ bath filled with Krebs-Henseleit solution. After 1 µg/ml lipopolysaccharide treatment, vasoconstrictors (either adrenaline or noradrenaline) were applied in a cumulative manner between concentrations of 10-9–10-5 mol/L of either prilocaine or mepivacaine (10-4 mol/L). Changes in isometric vasocontractions were continuously recorded with an amplifier system using the PCD-30A computer system. Results: The contractile response to both prilocaine and mepivacaine significantly decreased at low concentrations of adrenaline (10-8-10-7 mol/L). The contractile response to mepivacaine also significantly decreased at low concentrations of adrenaline (10-9-10-8 mol/L). In contrast, with the exception of prilocaine at a low concentration of adrenaline (10-8 mol/L), neither prilocaine nor mepivacaine significantly decreased the contractile responses. In a model of blood vessel inflammation under 6 h lipopolysaccharide treatment, prilocaine and mepivacaine enhanced contractile responses produced by 10-9-10-7 mol/L noradrenaline. Conclusion: Noradrenaline should be administered when injecting inflamed tissues with prilocaine or mepivacaine.

Original languageEnglish
Pages (from-to)3826-3831
Number of pages6
JournalBiomedical Research (India)
Volume28
Issue number9
Publication statusPublished - Jan 1 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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