Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer

Takeshi Iwaya, Takeo Fukagawa, Yutaka Suzuki, Yusuke Takahashi, Genta Sawada, Masahisa Ishibashi, Junji Kurashige, Tomoya Sudo, Fumiaki Tanaka, Kohei Shibata, Fumitaka Endo, Hirokatsu Katagiri, Kaoru Ishida, Kohei Kume, Satoshi Nishizuka, Hisae Iinuma, Go Wakabayashi, Masaki Mori, Mitsuru Sasako, Koshi Mimori

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Purpose: Recent studies revealed that both disseminated tumor cells and noncancerous cells contributed to cancer progression cooperatively in the bone marrow. Here, RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Experimental Design: Bone marrow samples from eight gastric cancer patients (stages I and IV: n = 4 each) were used for RNA-seq analysis. Results were validated through quantitative real-time PCR (qRT-PCR) analysis of HIST1H3D expression in 175 bone marrow, 92 peripheral blood, and 115 primary tumor samples from gastric cancer patients. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to confirm whether histone mRNAs were direct targets of miR-760. miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. Results: RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients was higher than that in stage I patients (P=0.0284, 0.0243, and 0.0006, respectively). In contrast, miR-760 was downregulated in the bone marrow and primary tumor of stage IV patients compared with stage I patients (P = 0.0094 and 0.0018, respectively). Histone mRNAand miR-760 interacted directly. Furthermore, miR-760 was downregulated in noncancerous mucosa in stage IV gastric cancer patients. Conclusion: Histone mRNA was upregulated, whereas miR-760 was downregulated in the bone marrow and primary tumor of advanced gastric cancer patients, suggesting that the histone mRNA/miR-760 axis had a crucial role in the development of gastric cancer.

Original languageEnglish
Pages (from-to)6438-6449
Number of pages12
JournalClinical Cancer Research
Volume19
Issue number23
DOIs
Publication statusPublished - Dec 1 2013

Fingerprint

MicroRNAs
Histones
Stomach Neoplasms
Messenger RNA
Bone Marrow
Neoplasms
Down-Regulation
RNA
Real-Time Polymerase Chain Reaction
Luciferases
Mucous Membrane
Research Design

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer. / Iwaya, Takeshi; Fukagawa, Takeo; Suzuki, Yutaka; Takahashi, Yusuke; Sawada, Genta; Ishibashi, Masahisa; Kurashige, Junji; Sudo, Tomoya; Tanaka, Fumiaki; Shibata, Kohei; Endo, Fumitaka; Katagiri, Hirokatsu; Ishida, Kaoru; Kume, Kohei; Nishizuka, Satoshi; Iinuma, Hisae; Wakabayashi, Go; Mori, Masaki; Sasako, Mitsuru; Mimori, Koshi.

In: Clinical Cancer Research, Vol. 19, No. 23, 01.12.2013, p. 6438-6449.

Research output: Contribution to journalArticle

Iwaya, T, Fukagawa, T, Suzuki, Y, Takahashi, Y, Sawada, G, Ishibashi, M, Kurashige, J, Sudo, T, Tanaka, F, Shibata, K, Endo, F, Katagiri, H, Ishida, K, Kume, K, Nishizuka, S, Iinuma, H, Wakabayashi, G, Mori, M, Sasako, M & Mimori, K 2013, 'Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer', Clinical Cancer Research, vol. 19, no. 23, pp. 6438-6449. https://doi.org/10.1158/1078-0432.CCR-12-3186
Iwaya, Takeshi ; Fukagawa, Takeo ; Suzuki, Yutaka ; Takahashi, Yusuke ; Sawada, Genta ; Ishibashi, Masahisa ; Kurashige, Junji ; Sudo, Tomoya ; Tanaka, Fumiaki ; Shibata, Kohei ; Endo, Fumitaka ; Katagiri, Hirokatsu ; Ishida, Kaoru ; Kume, Kohei ; Nishizuka, Satoshi ; Iinuma, Hisae ; Wakabayashi, Go ; Mori, Masaki ; Sasako, Mitsuru ; Mimori, Koshi. / Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer. In: Clinical Cancer Research. 2013 ; Vol. 19, No. 23. pp. 6438-6449.
@article{dfab6fa8c5e544bc83d4bb43dc896055,
title = "Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer",
abstract = "Purpose: Recent studies revealed that both disseminated tumor cells and noncancerous cells contributed to cancer progression cooperatively in the bone marrow. Here, RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Experimental Design: Bone marrow samples from eight gastric cancer patients (stages I and IV: n = 4 each) were used for RNA-seq analysis. Results were validated through quantitative real-time PCR (qRT-PCR) analysis of HIST1H3D expression in 175 bone marrow, 92 peripheral blood, and 115 primary tumor samples from gastric cancer patients. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to confirm whether histone mRNAs were direct targets of miR-760. miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. Results: RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients was higher than that in stage I patients (P=0.0284, 0.0243, and 0.0006, respectively). In contrast, miR-760 was downregulated in the bone marrow and primary tumor of stage IV patients compared with stage I patients (P = 0.0094 and 0.0018, respectively). Histone mRNAand miR-760 interacted directly. Furthermore, miR-760 was downregulated in noncancerous mucosa in stage IV gastric cancer patients. Conclusion: Histone mRNA was upregulated, whereas miR-760 was downregulated in the bone marrow and primary tumor of advanced gastric cancer patients, suggesting that the histone mRNA/miR-760 axis had a crucial role in the development of gastric cancer.",
author = "Takeshi Iwaya and Takeo Fukagawa and Yutaka Suzuki and Yusuke Takahashi and Genta Sawada and Masahisa Ishibashi and Junji Kurashige and Tomoya Sudo and Fumiaki Tanaka and Kohei Shibata and Fumitaka Endo and Hirokatsu Katagiri and Kaoru Ishida and Kohei Kume and Satoshi Nishizuka and Hisae Iinuma and Go Wakabayashi and Masaki Mori and Mitsuru Sasako and Koshi Mimori",
year = "2013",
month = "12",
day = "1",
doi = "10.1158/1078-0432.CCR-12-3186",
language = "English",
volume = "19",
pages = "6438--6449",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "23",

}

TY - JOUR

T1 - Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer

AU - Iwaya, Takeshi

AU - Fukagawa, Takeo

AU - Suzuki, Yutaka

AU - Takahashi, Yusuke

AU - Sawada, Genta

AU - Ishibashi, Masahisa

AU - Kurashige, Junji

AU - Sudo, Tomoya

AU - Tanaka, Fumiaki

AU - Shibata, Kohei

AU - Endo, Fumitaka

AU - Katagiri, Hirokatsu

AU - Ishida, Kaoru

AU - Kume, Kohei

AU - Nishizuka, Satoshi

AU - Iinuma, Hisae

AU - Wakabayashi, Go

AU - Mori, Masaki

AU - Sasako, Mitsuru

AU - Mimori, Koshi

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Purpose: Recent studies revealed that both disseminated tumor cells and noncancerous cells contributed to cancer progression cooperatively in the bone marrow. Here, RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Experimental Design: Bone marrow samples from eight gastric cancer patients (stages I and IV: n = 4 each) were used for RNA-seq analysis. Results were validated through quantitative real-time PCR (qRT-PCR) analysis of HIST1H3D expression in 175 bone marrow, 92 peripheral blood, and 115 primary tumor samples from gastric cancer patients. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to confirm whether histone mRNAs were direct targets of miR-760. miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. Results: RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients was higher than that in stage I patients (P=0.0284, 0.0243, and 0.0006, respectively). In contrast, miR-760 was downregulated in the bone marrow and primary tumor of stage IV patients compared with stage I patients (P = 0.0094 and 0.0018, respectively). Histone mRNAand miR-760 interacted directly. Furthermore, miR-760 was downregulated in noncancerous mucosa in stage IV gastric cancer patients. Conclusion: Histone mRNA was upregulated, whereas miR-760 was downregulated in the bone marrow and primary tumor of advanced gastric cancer patients, suggesting that the histone mRNA/miR-760 axis had a crucial role in the development of gastric cancer.

AB - Purpose: Recent studies revealed that both disseminated tumor cells and noncancerous cells contributed to cancer progression cooperatively in the bone marrow. Here, RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Experimental Design: Bone marrow samples from eight gastric cancer patients (stages I and IV: n = 4 each) were used for RNA-seq analysis. Results were validated through quantitative real-time PCR (qRT-PCR) analysis of HIST1H3D expression in 175 bone marrow, 92 peripheral blood, and 115 primary tumor samples from gastric cancer patients. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to confirm whether histone mRNAs were direct targets of miR-760. miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. Results: RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients was higher than that in stage I patients (P=0.0284, 0.0243, and 0.0006, respectively). In contrast, miR-760 was downregulated in the bone marrow and primary tumor of stage IV patients compared with stage I patients (P = 0.0094 and 0.0018, respectively). Histone mRNAand miR-760 interacted directly. Furthermore, miR-760 was downregulated in noncancerous mucosa in stage IV gastric cancer patients. Conclusion: Histone mRNA was upregulated, whereas miR-760 was downregulated in the bone marrow and primary tumor of advanced gastric cancer patients, suggesting that the histone mRNA/miR-760 axis had a crucial role in the development of gastric cancer.

UR - http://www.scopus.com/inward/record.url?scp=84890255361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890255361&partnerID=8YFLogxK

U2 - 10.1158/1078-0432.CCR-12-3186

DO - 10.1158/1078-0432.CCR-12-3186

M3 - Article

VL - 19

SP - 6438

EP - 6449

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 23

ER -