Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice

Kensuke Kusumi, Shoko Hirotsuka, Hiroshi Shimada, Yoko Chono, Osamu Matsuda, Koh Iba

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chloroplast biogenesis is most significant during the changes in cellular organization associated with leaf development in higher plants. To examine the physiological relationship between developing chloroplasts and host leaf cells during early leaf development, we investigated changes in the carbon and nitrogen contents in leaves at the P4 developmental stage of rice, during which leaf blade structure is established and early events of chloroplast differentiation occur. During the P4 stage, carbon content on a dry mass basis remained constant, whereas the nitrogen content decreased by 30%. Among carbohydrates, sucrose and starch accumulated to high levels early in the P4 stage, and glucose, fructose and cellulose degradation increased during the mid-to-late P4 stage. In the chloroplast-deficient leaves of the virescent-1 mutant of rice, however, the carbon and nitrogen contents, as well as the C/N ratio during the P4 stage, were largely unaffected. These observations suggest that developing rice leaves function as sink organs at the P4 stage, and that chloroplast biogenesis and carbon and nitrogen metabolism in the leaf cell is regulated independently at this stage.

Original languageEnglish
Pages (from-to)617-622
Number of pages6
JournalJournal of Plant Research
Volume123
Issue number4
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

leaf development
nitrogen balance
chloroplasts
rice
carbon
nitrogen content
leaves
nitrogen metabolism
leaf blade
carbon nitrogen ratio
fructose
cellulose
biogenesis
developmental stages
starch
sucrose
cells
carbohydrates
mutants
glucose

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice. / Kusumi, Kensuke; Hirotsuka, Shoko; Shimada, Hiroshi; Chono, Yoko; Matsuda, Osamu; Iba, Koh.

In: Journal of Plant Research, Vol. 123, No. 4, 01.01.2010, p. 617-622.

Research output: Contribution to journalArticle

@article{19e22cfb2ded4e4a8297d604303da9ac,
title = "Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice",
abstract = "Chloroplast biogenesis is most significant during the changes in cellular organization associated with leaf development in higher plants. To examine the physiological relationship between developing chloroplasts and host leaf cells during early leaf development, we investigated changes in the carbon and nitrogen contents in leaves at the P4 developmental stage of rice, during which leaf blade structure is established and early events of chloroplast differentiation occur. During the P4 stage, carbon content on a dry mass basis remained constant, whereas the nitrogen content decreased by 30{\%}. Among carbohydrates, sucrose and starch accumulated to high levels early in the P4 stage, and glucose, fructose and cellulose degradation increased during the mid-to-late P4 stage. In the chloroplast-deficient leaves of the virescent-1 mutant of rice, however, the carbon and nitrogen contents, as well as the C/N ratio during the P4 stage, were largely unaffected. These observations suggest that developing rice leaves function as sink organs at the P4 stage, and that chloroplast biogenesis and carbon and nitrogen metabolism in the leaf cell is regulated independently at this stage.",
author = "Kensuke Kusumi and Shoko Hirotsuka and Hiroshi Shimada and Yoko Chono and Osamu Matsuda and Koh Iba",
year = "2010",
month = "1",
day = "1",
doi = "10.1007/s10265-009-0277-x",
language = "English",
volume = "123",
pages = "617--622",
journal = "Journal of Plant Research",
issn = "0918-9440",
publisher = "Springer Japan",
number = "4",

}

TY - JOUR

T1 - Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice

AU - Kusumi, Kensuke

AU - Hirotsuka, Shoko

AU - Shimada, Hiroshi

AU - Chono, Yoko

AU - Matsuda, Osamu

AU - Iba, Koh

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Chloroplast biogenesis is most significant during the changes in cellular organization associated with leaf development in higher plants. To examine the physiological relationship between developing chloroplasts and host leaf cells during early leaf development, we investigated changes in the carbon and nitrogen contents in leaves at the P4 developmental stage of rice, during which leaf blade structure is established and early events of chloroplast differentiation occur. During the P4 stage, carbon content on a dry mass basis remained constant, whereas the nitrogen content decreased by 30%. Among carbohydrates, sucrose and starch accumulated to high levels early in the P4 stage, and glucose, fructose and cellulose degradation increased during the mid-to-late P4 stage. In the chloroplast-deficient leaves of the virescent-1 mutant of rice, however, the carbon and nitrogen contents, as well as the C/N ratio during the P4 stage, were largely unaffected. These observations suggest that developing rice leaves function as sink organs at the P4 stage, and that chloroplast biogenesis and carbon and nitrogen metabolism in the leaf cell is regulated independently at this stage.

AB - Chloroplast biogenesis is most significant during the changes in cellular organization associated with leaf development in higher plants. To examine the physiological relationship between developing chloroplasts and host leaf cells during early leaf development, we investigated changes in the carbon and nitrogen contents in leaves at the P4 developmental stage of rice, during which leaf blade structure is established and early events of chloroplast differentiation occur. During the P4 stage, carbon content on a dry mass basis remained constant, whereas the nitrogen content decreased by 30%. Among carbohydrates, sucrose and starch accumulated to high levels early in the P4 stage, and glucose, fructose and cellulose degradation increased during the mid-to-late P4 stage. In the chloroplast-deficient leaves of the virescent-1 mutant of rice, however, the carbon and nitrogen contents, as well as the C/N ratio during the P4 stage, were largely unaffected. These observations suggest that developing rice leaves function as sink organs at the P4 stage, and that chloroplast biogenesis and carbon and nitrogen metabolism in the leaf cell is regulated independently at this stage.

UR - http://www.scopus.com/inward/record.url?scp=77953958548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953958548&partnerID=8YFLogxK

U2 - 10.1007/s10265-009-0277-x

DO - 10.1007/s10265-009-0277-x

M3 - Article

C2 - 19953291

AN - SCOPUS:77953958548

VL - 123

SP - 617

EP - 622

JO - Journal of Plant Research

JF - Journal of Plant Research

SN - 0918-9440

IS - 4

ER -