Abstract
The involvement of FSH and triiodothyronine (T 3) in circadian clocks was investigated using immature granulosa cells of ovaries during the progress of cell maturation. Granulosa cells were prepared from preantral follicles of mouse Period2 (Per2)-dLuc reporter gene transgenic rats injected subcutaneously with the synthetic nonsteroidal estrogen diethylstilbestrol. Analysis of the cellular clock of the immature granulosa cells was performed partly using a serum-free culture system. Several bioluminescence oscillations of Per2-dLuc promoter activity were generated in the presence of FSH + fetal bovine serum, but not in the presence of either FSH or serum. As revealed by bioluminescence recording and analysis of clock gene expression, the granulosa cells lack the functional cellular clock at the immature stage, although Lhr was greatly expressed during the period of cell maturation. The granulosa cells gained a strong circadian rhythm of bioluminescence during stimulation with FSH, whereas LH reset the cellular clock of matured granulosa cells. During strong circadian rhythms of clock genes, the Star gene showed significant expression in matured granulosa cells. In contrast, T 3 showed an inhibitory effect on the development of the functional cellular clock during the period of cell maturation. These results indicate that FSH provides a cue for the development of the functional cellular clock of the immature granulosa cells, and T 3 blocks the development of the cellular clock.
| Original language | English |
|---|---|
| Pages (from-to) | 645-653 |
| Number of pages | 9 |
| Journal | American Journal of Physiology - Endocrinology and Metabolism |
| Volume | 302 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Mar 1 2012 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Physiology
- Physiology (medical)
Cite this
Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation. / Chu, Guiyan; Misawa, Izumi; Chen, Huatao; Yamauchi, Nobuhiko; Shigeyoshi, Yasufumi; Hashimoto, Seiichi; Hattori, Masa aki.
In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 302, No. 6, 01.03.2012, p. 645-653.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation
AU - Chu, Guiyan
AU - Misawa, Izumi
AU - Chen, Huatao
AU - Yamauchi, Nobuhiko
AU - Shigeyoshi, Yasufumi
AU - Hashimoto, Seiichi
AU - Hattori, Masa aki
PY - 2012/3/1
Y1 - 2012/3/1
N2 - The involvement of FSH and triiodothyronine (T 3) in circadian clocks was investigated using immature granulosa cells of ovaries during the progress of cell maturation. Granulosa cells were prepared from preantral follicles of mouse Period2 (Per2)-dLuc reporter gene transgenic rats injected subcutaneously with the synthetic nonsteroidal estrogen diethylstilbestrol. Analysis of the cellular clock of the immature granulosa cells was performed partly using a serum-free culture system. Several bioluminescence oscillations of Per2-dLuc promoter activity were generated in the presence of FSH + fetal bovine serum, but not in the presence of either FSH or serum. As revealed by bioluminescence recording and analysis of clock gene expression, the granulosa cells lack the functional cellular clock at the immature stage, although Lhr was greatly expressed during the period of cell maturation. The granulosa cells gained a strong circadian rhythm of bioluminescence during stimulation with FSH, whereas LH reset the cellular clock of matured granulosa cells. During strong circadian rhythms of clock genes, the Star gene showed significant expression in matured granulosa cells. In contrast, T 3 showed an inhibitory effect on the development of the functional cellular clock during the period of cell maturation. These results indicate that FSH provides a cue for the development of the functional cellular clock of the immature granulosa cells, and T 3 blocks the development of the cellular clock.
AB - The involvement of FSH and triiodothyronine (T 3) in circadian clocks was investigated using immature granulosa cells of ovaries during the progress of cell maturation. Granulosa cells were prepared from preantral follicles of mouse Period2 (Per2)-dLuc reporter gene transgenic rats injected subcutaneously with the synthetic nonsteroidal estrogen diethylstilbestrol. Analysis of the cellular clock of the immature granulosa cells was performed partly using a serum-free culture system. Several bioluminescence oscillations of Per2-dLuc promoter activity were generated in the presence of FSH + fetal bovine serum, but not in the presence of either FSH or serum. As revealed by bioluminescence recording and analysis of clock gene expression, the granulosa cells lack the functional cellular clock at the immature stage, although Lhr was greatly expressed during the period of cell maturation. The granulosa cells gained a strong circadian rhythm of bioluminescence during stimulation with FSH, whereas LH reset the cellular clock of matured granulosa cells. During strong circadian rhythms of clock genes, the Star gene showed significant expression in matured granulosa cells. In contrast, T 3 showed an inhibitory effect on the development of the functional cellular clock during the period of cell maturation. These results indicate that FSH provides a cue for the development of the functional cellular clock of the immature granulosa cells, and T 3 blocks the development of the cellular clock.
UR - http://www.scopus.com/inward/record.url?scp=84863276980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863276980&partnerID=8YFLogxK
U2 - 10.1152/ajpendo.00470.2011
DO - 10.1152/ajpendo.00470.2011
M3 - Article
C2 - 22205630
AN - SCOPUS:84863276980
VL - 302
SP - 645
EP - 653
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 6
ER -