Contribution of Gln9 and Phe80 to substrate binding in ribonuclease MC1 from bitter gourd seeds

Tomoyuki Numata, Makoto Kimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ribonuclease MC1 (RNase MC1) isolated from bitter gourd (Momordica charantia) seeds specifically cleaves phosphodiester bonds on the 5′-side of uridine. The crystal structures of RNase MC1 in complex with 2′-UMP or 3′-UMP reveal that Gln9, Asn71, Leu73, and Phe80 are involved in uridine binding by hydrogen bonding and hydrophobic interactions [Suzuki et al. (2000) Biochem. Biophys. Res. Commun. 275, 572 - 576]. To evaluate the contribution of Gln9 and Phe80 to uridine binding, Gln9 was replaced with Ala, Phe, Glu, or His, and Phe80 with Ala by site-directed mutagenesis. The kinetic properties of the resulting mutant enzymes were characterized using cytidylyl-3′,5′-uridine (CpU) as a substrate. The mutant Q9A exhibited a 3.7-fold increased Km and 27.6-fold decreased kcat, while three other mutations, Q9F, Q9E, and Q9H, predominantly affected the kcat value. Replacing Phe80 with Ala drastically reduced the catalytic efficiency (kcat/Km) with a minimum Km value equal to 8 mM. It was further found that the hydrolytic activities of the mutants toward cytidine-2′,3′-cyclic monophosphate (cCMP) were reduced. These results demonstrate that Gln9 and Phe80 play essential roles not only in uridine binding but also in hydrolytic activity. Moreover, we produced double Ala substituted mutants at Gln9, Asn71, Leu73, and Phe80, and compared their kinetic properties with those of the corresponding single mutants. The results suggest that these four residues may contribute to uridine binding in a mutually independent manner.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalJournal of Biochemistry
Volume130
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Momordica charantia
Uridine
Seed
Seeds
Uridine Monophosphate
Substrates
Cytidine
Mutagenesis
Kinetics
Hydrogen Bonding
Site-Directed Mutagenesis
Hydrophobic and Hydrophilic Interactions
Hydrogen bonds
Crystal structure
ribonuclease MC1
Mutation
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Contribution of Gln9 and Phe80 to substrate binding in ribonuclease MC1 from bitter gourd seeds. / Numata, Tomoyuki; Kimura, Makoto.

In: Journal of Biochemistry, Vol. 130, No. 5, 01.01.2001, p. 621-626.

Research output: Contribution to journalArticle

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