TY - JOUR
T1 - Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver
AU - Bamba, Takeshi
AU - Murayoshi, Michiko
AU - Gyoksen, Koichirou
AU - Nakazawa, Yoshihisa
AU - Okumoto, Hiroshi
AU - Katto, Hiroko
AU - Fukusaki, Eiichiro
AU - Kobayashi, Akio
PY - 2010
Y1 - 2010
N2 - The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the first time by feeding experiments using 13C-labeled isotopomers of (RS) mevalonate, 1-deoxy-D-xylulose- 3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13CNMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopentenyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisoprenoid; therefore, it was observed in the formation of low-molecular weight isoprenoids.
AB - The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the first time by feeding experiments using 13C-labeled isotopomers of (RS) mevalonate, 1-deoxy-D-xylulose- 3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13CNMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopentenyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisoprenoid; therefore, it was observed in the formation of low-molecular weight isoprenoids.
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U2 - 10.1515/znc-2010-5-608
DO - 10.1515/znc-2010-5-608
M3 - Article
C2 - 20653238
AN - SCOPUS:77954987990
SN - 0939-5075
VL - 65 C
SP - 363
EP - 372
JO - Zeitschrift fur Naturforschung. Section C: Biosciences
JF - Zeitschrift fur Naturforschung. Section C: Biosciences
IS - 5-6
ER -