Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver

Takeshi Bamba, Michiko Murayoshi, Koichirou Gyoksen, Yoshihisa Nakazawa, Hiroshi Okumoto, Hiroko Katto, Eiichiro Fukusaki, Akio Kobayashi

Research output: Contribution to journalArticle

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Abstract

The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the first time by feeding experiments using 13C-labeled isotopomers of (RS) mevalonate, 1-deoxy-D-xylulose- 3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13CNMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopentenyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisoprenoid; therefore, it was observed in the formation of low-molecular weight isoprenoids.

Original languageEnglish
Pages (from-to)363-372
Number of pages10
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume65 C
Issue number5-6
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

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Eucommiaceae
Mevalonic Acid
Biosynthesis
Rubber
Phosphates
Xylulose
Erythritol
Terpenes
Pyruvic Acid
Seedlings
Molecular Weight
Molecular weight
Nuclear magnetic resonance
isopentenyl pyrophosphate
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver. / Bamba, Takeshi; Murayoshi, Michiko; Gyoksen, Koichirou; Nakazawa, Yoshihisa; Okumoto, Hiroshi; Katto, Hiroko; Fukusaki, Eiichiro; Kobayashi, Akio.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 65 C, No. 5-6, 01.01.2010, p. 363-372.

Research output: Contribution to journalArticle

Bamba, Takeshi ; Murayoshi, Michiko ; Gyoksen, Koichirou ; Nakazawa, Yoshihisa ; Okumoto, Hiroshi ; Katto, Hiroko ; Fukusaki, Eiichiro ; Kobayashi, Akio. / Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2010 ; Vol. 65 C, No. 5-6. pp. 363-372.
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