Contribution of the transforming growth factor α B-loop β-sheet to binding and activation of the epidermal growth factor receptor

A. Richter, D. R. Drummond, J. MacGarvie, S. M. Puddicombe, S. G. Chamberlin, D. E. Davies

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have exploited the differences in binding affinities of the chicken epidermal growth factor (EGF) receptor for EGF and transforming growth factor a (TGFα) to study the role of the B-loop β-sheet of these ligands in receptor recognition and activation. Although EGF and TGFα share similar secondary and tertiary structures imposed by three highly conserved intramolecular disulfide bonds, they have only 30-40% overall sequence identity. The B-loop β-sheet is the major structural element in EGF and TGFα, but sequence similarity in this region is low. To investigate its role in receptor binding, we constructed two chimeric growth factors (mEGF/hTGFα21-30 and mEGFfhTGFα21-32) composed of the murine EGF (mEGF) amino acid sequence with residues 21-30 of the B-loop β-sheet replaced by the equivalent residues of human TGFα (hTGFα); in chimera mEGF/hTGFα31-32, asparagine 32, which lies at the boundary of the amino and carboxyl domains of mEGF, was also replaced by its hTGFα counterpart (valine). In initial studies using unpurified medium, it was found that the recombinant growth factors exhibited differing mitogenic potencies (mEGF/hTGFα21-32 > mEGF/hTGFα21-30 > mEGF) when assayed on chicken fibroblasts, even though they were equivalent in mitogenesis assays using cells expressing the human EGF receptor. After purification, mEGF/hTGFα21-32 was found to be 50 times more potent than mEGF in the chick fibroblast mitogenesis assay and exhibited a 10-fold increase in relative affinity for the chicken EGF receptor; both growth factors still exhibited equivalent mitogenic and receptor binding activity when tested on cells expressing human EGF receptors. We conclude that the B-loop β-sheet of hTGFα is an important determinant of EGF receptor binding affinity and biological activity.

Original languageEnglish
Pages (from-to)1612-1616
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number4
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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