Control of bacillus subtilis spores by intermittent treatment using heating after carbonation in the presence of germinants and bacteriostatic agents

Yukiko Tominaga, Seiji Noma, Noriyuki Igura, Mitsuya Shimoda, Nobuyuki Hayashi

Research output: Contribution to journalArticle

Abstract

We investigated the inactivation effect of intermittent treatments using carbonation under heating (CH) and second heating (HT), in the presence of germinants or monoglycerol caprate (MC10) on Bacillus subtilis spores. Intermittent treatments by CH, followed by incubation and a second HT, in the presence of germinants showed up to 2.6 log-order inactivation effects on spores. Compared with the effects of glycine, sodium benzoate, and potassium sorbate, MC10 increased the inactivation effect of CH. MC10 decreased the D value and activation energy for inactivation of the spores. Intermittent treatments with MC10, conducted by a CH, followed by HT, inactivated spores by 3.6 log-orders. CH with MC10 induced the release of dipicolinic acid (DPA) and increased the stainability of the spores by DAPI. This suggests that germinationlike phenomena, during CH, were included in the inactivation effects. Spores, which survived the intermittent treatment combined with MC10, did not proliferate in a liquid medium.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalFood Science and Technology Research
Volume24
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

carbonation
Carbonation
Bacilli
Bacillus subtilis
Spores
Heating
spores
heat
inactivation
Sorbic Acid
Potassium sorbate
Sodium Benzoate
sodium benzoate
potassium sorbate
activation energy
Glycine
Amino acids
Activation energy
Sodium

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

Control of bacillus subtilis spores by intermittent treatment using heating after carbonation in the presence of germinants and bacteriostatic agents. / Tominaga, Yukiko; Noma, Seiji; Igura, Noriyuki; Shimoda, Mitsuya; Hayashi, Nobuyuki.

In: Food Science and Technology Research, Vol. 24, No. 3, 01.01.2018, p. 403-411.

Research output: Contribution to journalArticle

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