Control of cytoskeletone structure by using 3D microporus film

Micro fabrication of cell culture substrates is one of the most significant subjects in the field of biomaterial research. Recently we found that endothelial cells can proliferate rapidly on a micro 3D patterned film (honeycomb film). The cell shape and cytoskeleton structure on the honeycomb films were clearly different from those on a flat film. In order to elucidate the effect of honeycomb films as a 3D scaffold for cell culture, it is needed that the 3D observation of cell behaviors such as the morphological change, expression of cytoskeleton, expression of contact points on extracellular adhesion molecules, and migration on the honeycomb films during cell culture. In this research, effects of 3D honeycomb pattern on above cell behaviors were observed.