TY - JOUR
T1 - Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film
AU - Tanaka, Masaru
AU - Nishikawa, Kazutaka
AU - Okubo, Hisashi
AU - Kamachi, Hirofumi
AU - Kawai, Tomoaki
AU - Matsushita, Michiaki
AU - Todo, Satoru
AU - Shimomura, Masatsugu
N1 - Funding Information:
This study is supported by grants-in-aid from Japan Science and Technology Corporation (JST) and Special Coordination Funds for Promoting Science and Technology.
PY - 2006/8/15
Y1 - 2006/8/15
N2 - Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices.
AB - Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices.
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U2 - 10.1016/j.colsurfa.2005.11.098
DO - 10.1016/j.colsurfa.2005.11.098
M3 - Article
AN - SCOPUS:33746904298
SN - 0927-7757
VL - 284-285
SP - 464
EP - 469
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
ER -