Control of neural stem cell differentiation on honeycomb films

Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 μm pore specifically.

Original languageEnglish
Pages (from-to)536-540
Number of pages5
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume313-314
DOIs
Publication statusPublished - Feb 1 2008
Externally publishedYes

Fingerprint

stem cells
Stem cells
porosity
Nestin
cerebral cortex
Polymer solutions
Tissue engineering
Pore size
tissue engineering
Atmospheric humidity
Casting
Neural networks
mice
humidity
Water
retarding
Substrates
polymers
water

All Science Journal Classification (ASJC) codes

  • Colloid and Surface Chemistry

Cite this

Control of neural stem cell differentiation on honeycomb films. / Tsuruma, Akinori; Tanaka, Masaru; Yamamoto, Sadaaki; Shimomura, Masatsugu.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 313-314, 01.02.2008, p. 536-540.

Research output: Contribution to journalArticle

Tsuruma, Akinori ; Tanaka, Masaru ; Yamamoto, Sadaaki ; Shimomura, Masatsugu. / Control of neural stem cell differentiation on honeycomb films. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2008 ; Vol. 313-314. pp. 536-540.
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