Control of the protein adsorption on honeycomb film by changing the pore size of honeycomb films

Micro fabrication of cell culture substrates is one of the most significant subjects in the field of biomaterial research. Recently we found that the proliferation of endothelial cells were influenced by the pore size of microporous polymer film (honeycomb film). It has been reported that adsorbed proteins on substrate play vital role in mediating cell proliferation. In order to elucidate the effect of the pore size of honeycomb films as a scaffold for cell culture, the observation of protein adsorption on honeycomb films during cell culture is needed. It was found the adsorbed protein structure on honeycomb films were greatly changed by changing pore size of honeycomb film. Further development of this approach should lead to surfaces which can control cell proliferation by changing the pore size of honeycomb films.