Abstract
In order to develop a microcapsule-shaped bioartificial pancreas, it is important to control the transport characteristics of the semipermeable membrane. We attempt to control the transport characteristics of the membrane by changing the coating layer number of polyelectrolyte complex between glycol chitosan (GC, M. W.>8.2×104) and alginate (Alg) on the microcapsule. From measurement of diffusivities of bovine serum albumin (BSA, M. W.= 6.7×104) and γ-globulin (M. W.=1.6×105), these diffusion rates through the membrane drastically decrease with increasing layer number of GC-Alg. The microcapsule coated with four layers of polyelectrolyte complex successfully rejects γ-globulin with effective permeability of BSA. In addition, the diffusivity of glucose (M. W.=180) indicateds that the multi-layering of polyelectrolyte complex hardly affects the diffusivity of small substances such as nutrients and oxygen. These results indicate that the polyelectrolyte complex prepared by the multi-layering method is useful to control the transport characteristic of membranes and the four-layered GC-Alg polyelectrolyte complex microcapsule is promising for a microcapsule-shaped bioartificial pancreas Key words: polyelectrolyte complex, multilayer coating, immunoisolation, bioartificial pancreas, microcapsule.
| Original language | English |
|---|---|
| Pages (from-to) | 165-168 |
| Number of pages | 4 |
| Journal | kagaku kogaku ronbunshu |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2001 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Control of Transport Characteristic of Membrane by Multi-layering of Polyelectrolyte Complex Toward Microcapsule-shaped Bioartificial Pancreas. / Saka, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Kofi.
In: kagaku kogaku ronbunshu, Vol. 27, No. 2, 01.01.2001, p. 165-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Control of Transport Characteristic of Membrane by Multi-layering of Polyelectrolyte Complex Toward Microcapsule-shaped Bioartificial Pancreas
AU - Saka, Shinji
AU - Ono, Tsutomu
AU - Ijima, Hiroyuki
AU - Kawakami, Kofi
PY - 2001/1/1
Y1 - 2001/1/1
N2 - In order to develop a microcapsule-shaped bioartificial pancreas, it is important to control the transport characteristics of the semipermeable membrane. We attempt to control the transport characteristics of the membrane by changing the coating layer number of polyelectrolyte complex between glycol chitosan (GC, M. W.>8.2×104) and alginate (Alg) on the microcapsule. From measurement of diffusivities of bovine serum albumin (BSA, M. W.= 6.7×104) and γ-globulin (M. W.=1.6×105), these diffusion rates through the membrane drastically decrease with increasing layer number of GC-Alg. The microcapsule coated with four layers of polyelectrolyte complex successfully rejects γ-globulin with effective permeability of BSA. In addition, the diffusivity of glucose (M. W.=180) indicateds that the multi-layering of polyelectrolyte complex hardly affects the diffusivity of small substances such as nutrients and oxygen. These results indicate that the polyelectrolyte complex prepared by the multi-layering method is useful to control the transport characteristic of membranes and the four-layered GC-Alg polyelectrolyte complex microcapsule is promising for a microcapsule-shaped bioartificial pancreas Key words: polyelectrolyte complex, multilayer coating, immunoisolation, bioartificial pancreas, microcapsule.
AB - In order to develop a microcapsule-shaped bioartificial pancreas, it is important to control the transport characteristics of the semipermeable membrane. We attempt to control the transport characteristics of the membrane by changing the coating layer number of polyelectrolyte complex between glycol chitosan (GC, M. W.>8.2×104) and alginate (Alg) on the microcapsule. From measurement of diffusivities of bovine serum albumin (BSA, M. W.= 6.7×104) and γ-globulin (M. W.=1.6×105), these diffusion rates through the membrane drastically decrease with increasing layer number of GC-Alg. The microcapsule coated with four layers of polyelectrolyte complex successfully rejects γ-globulin with effective permeability of BSA. In addition, the diffusivity of glucose (M. W.=180) indicateds that the multi-layering of polyelectrolyte complex hardly affects the diffusivity of small substances such as nutrients and oxygen. These results indicate that the polyelectrolyte complex prepared by the multi-layering method is useful to control the transport characteristic of membranes and the four-layered GC-Alg polyelectrolyte complex microcapsule is promising for a microcapsule-shaped bioartificial pancreas Key words: polyelectrolyte complex, multilayer coating, immunoisolation, bioartificial pancreas, microcapsule.
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U2 - 10.1252/kakoronbunshu.27.165
DO - 10.1252/kakoronbunshu.27.165
M3 - Article
VL - 27
SP - 165
EP - 168
JO - Kagaku Kogaku Ronbunshu
JF - Kagaku Kogaku Ronbunshu
SN - 0386-216X
IS - 2
ER -