Self-organized porous films (honeycomb films) with different pore size were prepared in order to investigate the influence of pore size on porcine arterial endothelial cells (PAECs) behavior. These films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly (ε-caprolactone)(PCL)) and an amphiphilic polymer. Four different pore sizes (5,10,12,16 μm) were prepared. Changes in cell morphology, proliferation, cytoskeleton and extra cellular matrix (ECM) (fibronectin, laminin, type IV collagen, elastin) production profiles were observed by optical, fluorescence, and scanning electron microscopy. The degree of spreading of PAECs was enhanced with increase of the pore size. PAECs proliferated on the honeycomb films. Almost the same ECM production profiles were obtained on the honeycomb films. These suggest that the honeycomb film is suitable as a material of a new vascular device.
|Number of pages||1|
|Publication status||Published - Dec 1 2005|
|Event||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
Duration: May 25 2005 → May 27 2005
|Other||54th SPSJ Annual Meeting 2005|
|Period||5/25/05 → 5/27/05|
All Science Journal Classification (ASJC) codes