Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene

Ko Ichi Oshiman, Kiyoto Motojima, Saleh Mahmood, Akira Shimada, Shigehiko Tamura, Masatomo Maeda, Masamitsu Futai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The rat gastric H+,K+-ATPase α subunit gene was cloned and the nucleotide sequence of its 5′-upstream region was determined. Sequence comparison with the corresponding part of the human gene indicated the presence of highly conserved regions which may be important for specific transcription of the α subunit in gastric parietal cells. The amino-terminal sequence (Met-Gly-Lys-Ala-Glu-) of the rat enzyme was similar to those of the pig and human enzymes. The gene organization of the rat enzyme was also similar to that of the human gene: introns 1, 2 and 9 were located in exactly the same positions as those in the human gene, and, as in the latter, exon 6 was not separated by an intron. The sequences of introns 1 and 2 were highly conserved among the rat, human and pig genes, but were entirely different from those of Na+,K+-ATPase catalytic subunit genes. Northern blot hybridization indicated that the gene was transcribed only in gastric mucosa.

Original languageEnglish
Pages (from-to)250-254
Number of pages5
JournalFEBS Letters
Volume281
Issue number1-2
DOIs
Publication statusPublished - Apr 9 1991
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Transcription
Adenosine Triphosphatases
Rats
Stomach
Genes
Introns
Enzymes
Swine
H(+)-K(+)-Exchanging ATPase
Gastric Parietal Cells
Gastric Mucosa
Northern Blotting
Exons
Catalytic Domain
Nucleotides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Oshiman, K. I., Motojima, K., Mahmood, S., Shimada, A., Tamura, S., Maeda, M., & Futai, M. (1991). Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene. FEBS Letters, 281(1-2), 250-254. https://doi.org/10.1016/0014-5793(91)80404-Q

Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene. / Oshiman, Ko Ichi; Motojima, Kiyoto; Mahmood, Saleh; Shimada, Akira; Tamura, Shigehiko; Maeda, Masatomo; Futai, Masamitsu.

In: FEBS Letters, Vol. 281, No. 1-2, 09.04.1991, p. 250-254.

Research output: Contribution to journalArticle

Oshiman, KI, Motojima, K, Mahmood, S, Shimada, A, Tamura, S, Maeda, M & Futai, M 1991, 'Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene', FEBS Letters, vol. 281, no. 1-2, pp. 250-254. https://doi.org/10.1016/0014-5793(91)80404-Q
Oshiman, Ko Ichi ; Motojima, Kiyoto ; Mahmood, Saleh ; Shimada, Akira ; Tamura, Shigehiko ; Maeda, Masatomo ; Futai, Masamitsu. / Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene. In: FEBS Letters. 1991 ; Vol. 281, No. 1-2. pp. 250-254.
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