Controlling the lectin recognition of glycopolymers: Via distance arrangement of sugar blocks

K. Jono, M. Nagao, T. Oh, S. Sonoda, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The arrangement of sugars in glycopolymers contributes to their recognition. The molecular recognition of proteins was controlled by the living radical polymerization of glycopolymers. The glycopolymers were prepared by the copolymerization of propargyl methacrylate (Pr-MA) and triethyleneglycol methacrylate (TEG-MA) via living radical polymerization with a reversible addition-fragmentation glycopolymer chain transfer (RAFT) reagent and by subsequent sugar conjugation by click chemistry. The block copolymers were prepared by the polymerization of Pr-MA and TEG-MA. The molecular recognition of glycopolymers was analyzed using the fluorescence quenching of lectin and found to be dependent on the glycopolymer structures. Two-site binding of glycopolymers to concanavalin A (ConA) was attained by both the glycopolymer with a 105-mer and the tri-block glycopolymer with a 103-mer. Glycopolymers with either a 27- or 54-mer showed much weaker interaction because of one-site binding. The molecular recognition of the glycopolymer was controlled by the arrangement and size of the sugar cluster and not by the sugar density.

Original languageEnglish
Pages (from-to)82-85
Number of pages4
JournalChemical Communications
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Methacrylates
Lectins
Sugars
Molecular recognition
Living polymerization
Binding sites
Free radical polymerization
Binding Sites
Concanavalin A
Copolymerization
Block copolymers
Quenching
Fluorescence
Polymerization
Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Controlling the lectin recognition of glycopolymers : Via distance arrangement of sugar blocks. / Jono, K.; Nagao, M.; Oh, T.; Sonoda, S.; Hoshino, Yu; Miura, Yoshiko.

In: Chemical Communications, Vol. 54, No. 1, 01.01.2017, p. 82-85.

Research output: Contribution to journalArticle

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