Conversion of arsenite and arsenate to methylarsenic and dimethylarsenic compounds by homogenates prepared from livers and kidneys of rats and mice

Miyuki Hirata, Takami Mohri, Akira Hisanaga, Noburu Ishinishi

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22 Citations (Scopus)

Abstract

Pooled livers and pooled kidneys from rats or mice were homogenized and spiked with arsenite or arsenate in the concentration range 1.3–20 μmol dm−3. Methylarsenic and dimethylarsenic compounds were determined by the hydride generation technique in the homogenates after a 90 min incubation at 37°C. The rat homogenates methylated arsenite and arsenate more efficiently than the mouse homogenates. Monomethylated arsenic was present in larger amounts than dimethylated arsenic in the rat homogenates. In the absence of reduced glutathione (GSH), no methylation occurred. Addition of GSH promoted monomethylation and dimethylation, whereas dithiothreitol and mercaptoethanol (10 mmol dm−3) fostered only monomethylation. The amounts of monomethylated arsenic in the rat liver homogenates increased with increasing arsenite concentration (1.3–20 μmol dm−3) however, the percentage of arsenic that had been methylated decreased. A similar trend, but with much less monomethylarsenic formed, was observed for arsenate‐spiked homogenates. Rat kidney homogenates methylated arsenite and arsenate to a much smaller extent than rat liver homogenates. The Km values for the monomethylation in rat liver homogenates were found to be 5.3 μmol dm−3 for arsenite and 59 μmol dm−3 for arsenate.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalApplied Organometallic Chemistry
Volume3
Issue number4
DOIs
Publication statusPublished - 1989

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Inorganic Chemistry

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