Conversion of Nitrite of Nitrate by Nitrite-Resistant Yeasts

Takashi Tachiki; Kenji Sakai; Katsu Yamamoto; Masayuki Hatanaka; Tatsurokuro Tochikura

doi:10.1271/bbb1961.52.1999

Conversion of Nitrite of Nitrate by Nitrite-Resistant Yeasts

Takashi Tachiki, Kenji Sakai, Katsu Yamamoto, Masayuki Hatanaka, Tatsurokuro Tochikura

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Candida species YK 11 and YK 92 and Geotrichum candidum YK 57, which were isolated as nitrite-resistants, converted nitrite in the culture medium to nitrate stoichiometrically during growth. The nitrite-oxidizing reaction was confirmed under aerobic conditions in the intact cell system with 15 mMnitrite, 150 mm glucose, and 100 mm Tris-HCl buffer (pH 7.0). Glucose or other carbohydrate which supported the microbial growth was indispensable for the reaction. The rate of oxidation (0.9~1.3 } 10⁵ µg-N/g of YK 92 cells-day) and the maximum amounts of nitrate formed in the culture medium (200 mm, 2800 µg-N/ml) were much larger than those of other heterotrophic nitrifiers and almost the same as those of Nitrobacter. The nitrite-oxidizing activity was demonstrated in many types of yeast species.

Original language	English
Pages (from-to)	1999-2005
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	52
Issue number	8
DOIs	https://doi.org/10.1271/bbb1961.52.1999
Publication status	Published - 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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title = "Conversion of Nitrite of Nitrate by Nitrite-Resistant Yeasts",

abstract = "Candida species YK 11 and YK 92 and Geotrichum candidum YK 57, which were isolated as nitrite-resistants, converted nitrite in the culture medium to nitrate stoichiometrically during growth. The nitrite-oxidizing reaction was confirmed under aerobic conditions in the intact cell system with 15 mMnitrite, 150 mm glucose, and 100 mm Tris-HCl buffer (pH 7.0). Glucose or other carbohydrate which supported the microbial growth was indispensable for the reaction. The rate of oxidation (0.9~1.3 } 105 µg-N/g of YK 92 cells-day) and the maximum amounts of nitrate formed in the culture medium (200 mm, 2800 µg-N/ml) were much larger than those of other heterotrophic nitrifiers and almost the same as those of Nitrobacter. The nitrite-oxidizing activity was demonstrated in many types of yeast species.",

author = "Takashi Tachiki and Kenji Sakai and Katsu Yamamoto and Masayuki Hatanaka and Tatsurokuro Tochikura",

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doi = "10.1271/bbb1961.52.1999",

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T1 - Conversion of Nitrite of Nitrate by Nitrite-Resistant Yeasts

AU - Tachiki, Takashi

AU - Sakai, Kenji

AU - Yamamoto, Katsu

AU - Hatanaka, Masayuki

AU - Tochikura, Tatsurokuro

PY - 1988

Y1 - 1988

N2 - Candida species YK 11 and YK 92 and Geotrichum candidum YK 57, which were isolated as nitrite-resistants, converted nitrite in the culture medium to nitrate stoichiometrically during growth. The nitrite-oxidizing reaction was confirmed under aerobic conditions in the intact cell system with 15 mMnitrite, 150 mm glucose, and 100 mm Tris-HCl buffer (pH 7.0). Glucose or other carbohydrate which supported the microbial growth was indispensable for the reaction. The rate of oxidation (0.9~1.3 } 105 µg-N/g of YK 92 cells-day) and the maximum amounts of nitrate formed in the culture medium (200 mm, 2800 µg-N/ml) were much larger than those of other heterotrophic nitrifiers and almost the same as those of Nitrobacter. The nitrite-oxidizing activity was demonstrated in many types of yeast species.

AB - Candida species YK 11 and YK 92 and Geotrichum candidum YK 57, which were isolated as nitrite-resistants, converted nitrite in the culture medium to nitrate stoichiometrically during growth. The nitrite-oxidizing reaction was confirmed under aerobic conditions in the intact cell system with 15 mMnitrite, 150 mm glucose, and 100 mm Tris-HCl buffer (pH 7.0). Glucose or other carbohydrate which supported the microbial growth was indispensable for the reaction. The rate of oxidation (0.9~1.3 } 105 µg-N/g of YK 92 cells-day) and the maximum amounts of nitrate formed in the culture medium (200 mm, 2800 µg-N/ml) were much larger than those of other heterotrophic nitrifiers and almost the same as those of Nitrobacter. The nitrite-oxidizing activity was demonstrated in many types of yeast species.

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Conversion of Nitrite of Nitrate by Nitrite-Resistant Yeasts

Abstract

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