Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions

Go Nagamatsu, Toshio Suda

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.

Original languageEnglish
Title of host publicationImaging and Tracking Stem Cells
Subtitle of host publicationMethods and Protocols
Pages49-56
Number of pages8
DOIs
Publication statusPublished - Sep 4 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1052
ISSN (Print)1064-3745

Fingerprint

Cell Tracking
Pluripotent Stem Cells
Germ Cells
Cell Culture Techniques
Fertilization
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Nagamatsu, G., & Suda, T. (2013). Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. In Imaging and Tracking Stem Cells: Methods and Protocols (pp. 49-56). (Methods in Molecular Biology; Vol. 1052). https://doi.org/10.1007/7651-2013-24

Conversion of primordial germ cells to pluripotent stem cells : Methods for cell tracking and culture conditions. / Nagamatsu, Go; Suda, Toshio.

Imaging and Tracking Stem Cells: Methods and Protocols. 2013. p. 49-56 (Methods in Molecular Biology; Vol. 1052).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nagamatsu, G & Suda, T 2013, Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. in Imaging and Tracking Stem Cells: Methods and Protocols. Methods in Molecular Biology, vol. 1052, pp. 49-56. https://doi.org/10.1007/7651-2013-24
Nagamatsu G, Suda T. Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. In Imaging and Tracking Stem Cells: Methods and Protocols. 2013. p. 49-56. (Methods in Molecular Biology). https://doi.org/10.1007/7651-2013-24
Nagamatsu, Go ; Suda, Toshio. / Conversion of primordial germ cells to pluripotent stem cells : Methods for cell tracking and culture conditions. Imaging and Tracking Stem Cells: Methods and Protocols. 2013. pp. 49-56 (Methods in Molecular Biology).
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