Conversion of Salmonella typhimurium to L-forms contributes to the maintenance of acquired immunity against murine typhoid

E. Kita, M. Emoto, F. Nishikawa, Y. Yoshikai, S. Kashiba

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Conversion of Salmonella typhimurium to L-forms, both in vitro and in vivo, resulted in the expression of proteins cross-reacting to the mycobacterial 65 000 MW heat-shock protein (hsp). Immunization of C3H/HeJ mice with a protective dose of stable L-form S. typhimurium induced γδ T cells in the liver, in accordance with the multiplication of L-form Salmonella in Kupffer cells. The number of γδ T cells decreased after the intracellular growth of L-form Salmonella plateaued. Persistance of the L-forms in Kupffer cells, however, allowed hepatic γδ T cells to increase within 48 hr of infection with virulent S. typhimurium. Thus, the intrahepatic colonization of L-form Salmonella seems to keep γδ T cells on standby, but the emergence of these T cells does not correlate with the expression of L-form hsp. In addition, Kupffer cells colonized by L-forms constitutively synthesized mRNA for interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α). These results suggest that conversion of S. typhimurium to L-forms in phagocytic cells builds up and maintains acquired resistance, conferred by live-cell vaccines of S. typhimurium, against murine typhoid.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalImmunology
Volume86
Issue number2
Publication statusPublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology

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