We report that short-chain ceramide (Cer), C2- and C6Cer, were immediately glycosylated and finally converted to short-chain Cer GM3 in B16 melanoma cells. By addition of either C2- or C6Cer to a cell culture of B16 melanoma in the presence of [14C]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs. These novel GSLs were identified as C2-, C6Cer cerebrosides and C2-, C6Cer GM3, respectively. In comparison with C2Cer, C6Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short-chain Cer.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology