Coordinated design of cofactor and active site structures in development of new protein catalysts

Takafumi Ueno, Tomomi Koshiyama, Masataka Ohashi, Kazuyoshi Kondo, Masaharu Kono, Atsuo Suzuki, Takashi Yamane, Yoshihito Watanabe

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146 Citations (Scopus)

Abstract

New methods for the synthesis of artificial metalloenzymes are important for the construction of novel biocatalysts and biomaterials. Recently, we reported new methodology for the synthesis of artificial metalloenzymes by reconstituting apo-myoglobin with metal complexes (Ohashi, M. et al., Angew Chem., Int. Ed. 2003, 42, 1005-1008). However, it has been difficult to improve their reactivity, since their crystal structures were not available. In this article, we report the crystal structures of MIII(Schiff base)·apo-A71GMbs (M = Cr and Mn). The structures suggest that the position of the metal complex in apo-Mb is regulated by (i) noncovalent interaction between the ligand and surrounding peptides and (ii) the ligation of the metal ion to proximal histidine (His93). In addition, it is proposed that specific interactions of Ile107 with 3- and 3′-substituent groups on the salen ligand control the location of the Schiff base ligand in the active site. On the basis of these results, we have successfully controlled the enantioselectivity in the sulfoxidation of thioanisole by changing the size of substituents at the 3 and 3′ positions. This is the first example of an enantioselective enzymatic reaction regulated by the design of metal complex in the protein active site.

Original languageEnglish
Pages (from-to)6556-6562
Number of pages7
JournalJournal of the American Chemical Society
Volume127
Issue number18
DOIs
Publication statusPublished - May 11 2005

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All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Ueno, T., Koshiyama, T., Ohashi, M., Kondo, K., Kono, M., Suzuki, A., ... Watanabe, Y. (2005). Coordinated design of cofactor and active site structures in development of new protein catalysts. Journal of the American Chemical Society, 127(18), 6556-6562. https://doi.org/10.1021/ja045995q