Coordinated leading and lagging strand DNA synthesis on a minicircular template

Joonsoo Lee, Paul D. Chastain, Takahiro Kusakabe, Jack D. Griffith, Charles C. Richardson

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled : (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.

Original languageEnglish
Pages (from-to)1001-1010
Number of pages10
JournalMolecular Cell
Volume1
Issue number7
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

DNA-Binding Proteins
DNA-Directed DNA Polymerase
DNA Primase
DNA Helicases
Bacteriophage T7
DNA
Proteins
Okazaki fragments
Direction compound

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Coordinated leading and lagging strand DNA synthesis on a minicircular template. / Lee, Joonsoo; Chastain, Paul D.; Kusakabe, Takahiro; Griffith, Jack D.; Richardson, Charles C.

In: Molecular Cell, Vol. 1, No. 7, 01.01.1998, p. 1001-1010.

Research output: Contribution to journalArticle

Lee, Joonsoo ; Chastain, Paul D. ; Kusakabe, Takahiro ; Griffith, Jack D. ; Richardson, Charles C. / Coordinated leading and lagging strand DNA synthesis on a minicircular template. In: Molecular Cell. 1998 ; Vol. 1, No. 7. pp. 1001-1010.
@article{e43f2081600c44eab020fa990f4c9f1a,
title = "Coordinated leading and lagging strand DNA synthesis on a minicircular template",
abstract = "The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled : (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.",
author = "Joonsoo Lee and Chastain, {Paul D.} and Takahiro Kusakabe and Griffith, {Jack D.} and Richardson, {Charles C.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1016/S1097-2765(00)80100-8",
language = "English",
volume = "1",
pages = "1001--1010",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "7",

}

TY - JOUR

T1 - Coordinated leading and lagging strand DNA synthesis on a minicircular template

AU - Lee, Joonsoo

AU - Chastain, Paul D.

AU - Kusakabe, Takahiro

AU - Griffith, Jack D.

AU - Richardson, Charles C.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled : (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.

AB - The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled : (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.

UR - http://www.scopus.com/inward/record.url?scp=0032086491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032086491&partnerID=8YFLogxK

U2 - 10.1016/S1097-2765(00)80100-8

DO - 10.1016/S1097-2765(00)80100-8

M3 - Article

C2 - 9651583

AN - SCOPUS:0032086491

VL - 1

SP - 1001

EP - 1010

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 7

ER -