Coordinated regulation by two VPS9 domain-containing guanine nucleotide exchange factors in small GTPase Rab5 signaling pathways in fission yeast

Yuta Tsukamoto, Satoshi Kagiwada, Sayuri Shimazu, Kaoru Takegawa, Tetsuko Noguchi, Masaaki Miyamoto

Research output: Contribution to journalArticle

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Abstract

Abstract The small GTPase Rab5 is reported to regulate various cellular functions, such as vesicular transport and endocytosis. VPS9 domain-containing proteins are thought to activate Rab5(s) by their guanine-nucleotide exchange activities. Numerous VPS9 proteins have been identified and are structurally conserved from yeast to mammalian cells. However, the functional relationships among VPS9 proteins in cells remain unclear. Only one Rab5 and two VPS9 proteins were identified in the Schizosaccharomyces pombe genome. Here, we examined the cellular function of two VPS9 proteins and the relationship between these proteins in cellular functions. Vps901-GFP and Vps902-GFP exhibited dotted signals in vegetative and differentiated cells. vps901 deletion mutant (Δvps901) cells exhibited a phenotype deficient in the mating process and responses to high concentrations of ions, such as calcium and metals, and Δvps901Δvps902 double mutant cells exhibited round cell shapes similar to ypt5-909 (Rab5 mutant allele) cells. Deletion of both vps901 and vps902 genes completely abolished the mating process and responses to various stresses. A lack of vacuole formation and aberrant inner cell membrane structures were also observed in Δvps901Δvps902 cells by electron microscopy. These data strongly suggest that Vps901 and Vps902 are cooperatively involved in the regulation of cellular functions, such as cell morphology, sexual development, response to ion stresses, and vacuole formation, via Rab5 signaling pathways in fission yeast cells.

Original languageEnglish
Article number33423
Pages (from-to)802-809
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume458
Issue number4
DOIs
Publication statusPublished - Mar 20 2015

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Guanine Nucleotide Exchange Factors
Monomeric GTP-Binding Proteins
Schizosaccharomyces
Yeast
Proteins
Genes
Cells
Ions
Vacuoles
Membrane structures
Guanine Nucleotides
Cell membranes
Cell Membrane Structures
Electron microscopy
Sexual Development
Cell Shape
Metals
Endocytosis
Calcium
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Coordinated regulation by two VPS9 domain-containing guanine nucleotide exchange factors in small GTPase Rab5 signaling pathways in fission yeast. / Tsukamoto, Yuta; Kagiwada, Satoshi; Shimazu, Sayuri; Takegawa, Kaoru; Noguchi, Tetsuko; Miyamoto, Masaaki.

In: Biochemical and Biophysical Research Communications, Vol. 458, No. 4, 33423, 20.03.2015, p. 802-809.

Research output: Contribution to journalArticle

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