Coordinated regulation of palladin and α-smooth muscle actin by transforming growth factor-β in human corneal fibroblasts

Naoyuki Morishige, Shizuka Murata, Yoshikuni Nakamura, Haruya Azumi, Ryutaro Shin-Gyou-Uchi, Ken Taro Oki, Yukiko Morita, Koh Hei Sonoda

Research output: Contribution to journalArticle

Abstract

PURPOSE. To investigate the role of palladin in the cornea, we examined expression of this actin assembly-related protein in normal, diseased, or injured corneal tissue as well as in cultured corneal fibroblasts. METHODS. Expression of palladin and α-smooth muscle actin (α-SMA) in the rat cornea with an incision wound, in the normal and diseased human cornea, and in cultured human corneal fibroblasts was examined by immunofluorescence or immunoblot analysis. RESULTS. The expression of both palladin and α-SMA was detected at the lesion site during wound healing in the rat cornea. Whereas neither palladin nor α-SMA was detected in the normal human cornea, the colocalization of both proteins was detected in diseased human corneas with underlying conditions characterized by the presence of fibrosis. The expression of both palladin and α-SMA in cultured human corneal fibroblasts was increased by transforming growth factor-β (TGF-β) in a manner sensitive to inhibition by blockers of Smad or mitogen-activated protein kinase (MAPK) signaling. Finally, RNA interference-mediated depletion of palladin attenuated the TGF-β-induced upregulation of α-SMA expression in human corneal fibroblasts as well as TGF-β-induced collagen gel contraction mediated by these cells. CONCLUSIONS. Palladin is expressed in the rat and human cornea in association with scar formation. Expression of palladin in human corneal fibroblasts is increased by TGF-β in a manner dependent on Smad and MAPK signaling and is required for the TGF-β-induced upregulation of α-SMA.

Original languageEnglish
Pages (from-to)3360-3368
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number7
DOIs
Publication statusPublished - Jun 1 2016
Externally publishedYes

Fingerprint

Transforming Growth Factors
Smooth Muscle
Actins
Cornea
Fibroblasts
Mitogen-Activated Protein Kinases
Up-Regulation
Fibroblast Growth Factors
RNA Interference
Wound Healing
Cicatrix
Fluorescent Antibody Technique
Proteins
Fibrosis
Collagen
Gels
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Coordinated regulation of palladin and α-smooth muscle actin by transforming growth factor-β in human corneal fibroblasts. / Morishige, Naoyuki; Murata, Shizuka; Nakamura, Yoshikuni; Azumi, Haruya; Shin-Gyou-Uchi, Ryutaro; Oki, Ken Taro; Morita, Yukiko; Sonoda, Koh Hei.

In: Investigative Ophthalmology and Visual Science, Vol. 57, No. 7, 01.06.2016, p. 3360-3368.

Research output: Contribution to journalArticle

Morishige, Naoyuki ; Murata, Shizuka ; Nakamura, Yoshikuni ; Azumi, Haruya ; Shin-Gyou-Uchi, Ryutaro ; Oki, Ken Taro ; Morita, Yukiko ; Sonoda, Koh Hei. / Coordinated regulation of palladin and α-smooth muscle actin by transforming growth factor-β in human corneal fibroblasts. In: Investigative Ophthalmology and Visual Science. 2016 ; Vol. 57, No. 7. pp. 3360-3368.
@article{ec5d25dbc6724784a4545e3360070598,
title = "Coordinated regulation of palladin and α-smooth muscle actin by transforming growth factor-β in human corneal fibroblasts",
abstract = "PURPOSE. To investigate the role of palladin in the cornea, we examined expression of this actin assembly-related protein in normal, diseased, or injured corneal tissue as well as in cultured corneal fibroblasts. METHODS. Expression of palladin and α-smooth muscle actin (α-SMA) in the rat cornea with an incision wound, in the normal and diseased human cornea, and in cultured human corneal fibroblasts was examined by immunofluorescence or immunoblot analysis. RESULTS. The expression of both palladin and α-SMA was detected at the lesion site during wound healing in the rat cornea. Whereas neither palladin nor α-SMA was detected in the normal human cornea, the colocalization of both proteins was detected in diseased human corneas with underlying conditions characterized by the presence of fibrosis. The expression of both palladin and α-SMA in cultured human corneal fibroblasts was increased by transforming growth factor-β (TGF-β) in a manner sensitive to inhibition by blockers of Smad or mitogen-activated protein kinase (MAPK) signaling. Finally, RNA interference-mediated depletion of palladin attenuated the TGF-β-induced upregulation of α-SMA expression in human corneal fibroblasts as well as TGF-β-induced collagen gel contraction mediated by these cells. CONCLUSIONS. Palladin is expressed in the rat and human cornea in association with scar formation. Expression of palladin in human corneal fibroblasts is increased by TGF-β in a manner dependent on Smad and MAPK signaling and is required for the TGF-β-induced upregulation of α-SMA.",
author = "Naoyuki Morishige and Shizuka Murata and Yoshikuni Nakamura and Haruya Azumi and Ryutaro Shin-Gyou-Uchi and Oki, {Ken Taro} and Yukiko Morita and Sonoda, {Koh Hei}",
year = "2016",
month = "6",
day = "1",
doi = "10.1167/iovs.15-18763",
language = "English",
volume = "57",
pages = "3360--3368",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "7",

}

TY - JOUR

T1 - Coordinated regulation of palladin and α-smooth muscle actin by transforming growth factor-β in human corneal fibroblasts

AU - Morishige, Naoyuki

AU - Murata, Shizuka

AU - Nakamura, Yoshikuni

AU - Azumi, Haruya

AU - Shin-Gyou-Uchi, Ryutaro

AU - Oki, Ken Taro

AU - Morita, Yukiko

AU - Sonoda, Koh Hei

PY - 2016/6/1

Y1 - 2016/6/1

N2 - PURPOSE. To investigate the role of palladin in the cornea, we examined expression of this actin assembly-related protein in normal, diseased, or injured corneal tissue as well as in cultured corneal fibroblasts. METHODS. Expression of palladin and α-smooth muscle actin (α-SMA) in the rat cornea with an incision wound, in the normal and diseased human cornea, and in cultured human corneal fibroblasts was examined by immunofluorescence or immunoblot analysis. RESULTS. The expression of both palladin and α-SMA was detected at the lesion site during wound healing in the rat cornea. Whereas neither palladin nor α-SMA was detected in the normal human cornea, the colocalization of both proteins was detected in diseased human corneas with underlying conditions characterized by the presence of fibrosis. The expression of both palladin and α-SMA in cultured human corneal fibroblasts was increased by transforming growth factor-β (TGF-β) in a manner sensitive to inhibition by blockers of Smad or mitogen-activated protein kinase (MAPK) signaling. Finally, RNA interference-mediated depletion of palladin attenuated the TGF-β-induced upregulation of α-SMA expression in human corneal fibroblasts as well as TGF-β-induced collagen gel contraction mediated by these cells. CONCLUSIONS. Palladin is expressed in the rat and human cornea in association with scar formation. Expression of palladin in human corneal fibroblasts is increased by TGF-β in a manner dependent on Smad and MAPK signaling and is required for the TGF-β-induced upregulation of α-SMA.

AB - PURPOSE. To investigate the role of palladin in the cornea, we examined expression of this actin assembly-related protein in normal, diseased, or injured corneal tissue as well as in cultured corneal fibroblasts. METHODS. Expression of palladin and α-smooth muscle actin (α-SMA) in the rat cornea with an incision wound, in the normal and diseased human cornea, and in cultured human corneal fibroblasts was examined by immunofluorescence or immunoblot analysis. RESULTS. The expression of both palladin and α-SMA was detected at the lesion site during wound healing in the rat cornea. Whereas neither palladin nor α-SMA was detected in the normal human cornea, the colocalization of both proteins was detected in diseased human corneas with underlying conditions characterized by the presence of fibrosis. The expression of both palladin and α-SMA in cultured human corneal fibroblasts was increased by transforming growth factor-β (TGF-β) in a manner sensitive to inhibition by blockers of Smad or mitogen-activated protein kinase (MAPK) signaling. Finally, RNA interference-mediated depletion of palladin attenuated the TGF-β-induced upregulation of α-SMA expression in human corneal fibroblasts as well as TGF-β-induced collagen gel contraction mediated by these cells. CONCLUSIONS. Palladin is expressed in the rat and human cornea in association with scar formation. Expression of palladin in human corneal fibroblasts is increased by TGF-β in a manner dependent on Smad and MAPK signaling and is required for the TGF-β-induced upregulation of α-SMA.

UR - http://www.scopus.com/inward/record.url?scp=84976384864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976384864&partnerID=8YFLogxK

U2 - 10.1167/iovs.15-18763

DO - 10.1167/iovs.15-18763

M3 - Article

C2 - 27367503

AN - SCOPUS:84976384864

VL - 57

SP - 3360

EP - 3368

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 7

ER -