Abstract
To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.
| Original language | English |
|---|---|
| Pages (from-to) | 773-775 |
| Number of pages | 3 |
| Journal | Analytical Biochemistry |
| Volume | 421 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 15 2012 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay. / Tsuchiya, Akira; Asai, Daisuke; Kang, Jeong Hun; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.
In: Analytical Biochemistry, Vol. 421, No. 2, 15.02.2012, p. 773-775.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay
AU - Tsuchiya, Akira
AU - Asai, Daisuke
AU - Kang, Jeong Hun
AU - Mori, Takeshi
AU - Niidome, Takuro
AU - Katayama, Yoshiki
PY - 2012/2/15
Y1 - 2012/2/15
N2 - To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.
AB - To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.
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UR - http://www.scopus.com/inward/citedby.url?scp=84859718536&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2011.08.035
DO - 10.1016/j.ab.2011.08.035
M3 - Article
C2 - 21925483
AN - SCOPUS:84859718536
VL - 421
SP - 773
EP - 775
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -