Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay

Akira Tsuchiya, Daisuke Asai, Jeong Hun Kang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.

Original languageEnglish
Pages (from-to)773-775
Number of pages3
JournalAnalytical Biochemistry
Volume421
Issue number2
DOIs
Publication statusPublished - Feb 15 2012

Fingerprint

Phosphorylation
Radioactivity
Ionization
Mass spectrometry
Assays
Desorption
Mass Spectrometry
Lasers
Amino Acids
Peptides
Protein Kinases
Monitoring
Substrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.",
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AU - Asai, Daisuke

AU - Kang, Jeong Hun

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Katayama, Yoshiki

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