Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay

Akira Tsuchiya; Daisuke Asai; Jeong Hun Kang; Takeshi Mori; Takuro Niidome; Yoshiki Katayama

doi:10.1016/j.ab.2011.08.035

Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay

Akira Tsuchiya, Daisuke Asai, Jeong Hun Kang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Oral Rehabilitation

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.

Original language	English
Pages (from-to)	773-775
Number of pages	3
Journal	Analytical Biochemistry
Volume	421
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2011.08.035
Publication status	Published - Feb 15 2012

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay",

abstract = "To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.",

author = "Akira Tsuchiya and Daisuke Asai and Kang, {Jeong Hun} and Takeshi Mori and Takuro Niidome and Yoshiki Katayama",

note = "Funding Information: This work was financially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan . ",

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AU - Tsuchiya, Akira

AU - Asai, Daisuke

AU - Kang, Jeong Hun

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Katayama, Yoshiki

N1 - Funding Information: This work was financially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan .

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Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay

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