Correlation of partition coefficients of enzymes and amino acids in dextran+poly (ethylene glycol)+water aqueous two-phase systems

Fan Zhou; Sunao Yamada; Satien Dew Raghoe; Keijiro Kakisaka; Takeshi Furuya; Yoshio Iwai; Yasuhiko Arai

Correlation of partition coefficients of enzymes and amino acids in dextran+poly (ethylene glycol)+water aqueous two-phase systems

Fan Zhou, Sunao Yamada, Satien Dew Raghoe, Keijiro Kakisaka, Takeshi Furuya, Yoshio Iwai, Yasuhiko Arai

Research output: Contribution to journal › Article › peer-review

Abstract

The partition coefficients of hydrolytic enzymes (α-amylase, β-amylase and glucoamylase) and amino acids (glycine, phenylalanine and isoleucine) in dextran+poly (ethylene glycol)+water aqueous two-phase systems were correlated by using the osmotic virial equation, the conventional Flory-Huggins equation, and a modified Flory-Huggins equation which were empirically proposed for aqueous polymer solutions, and their correlation performance are presented. Moreover, the effects of the molecular weights and the concentrations of polymers on the partition coefficients were also discussed. The interaction parameters required were determined by fitting the equations to the experimental partition coefficient data and good agreement was obtained.

Original language	English
Pages (from-to)	69-82
Number of pages	14
Journal	Memoirs of the Kyushu University, Faculty of Engineering
Volume	56
Issue number	2
Publication status	Published - Jun 1 1996

All Science Journal Classification (ASJC) codes

Energy(all)
Atmospheric Science
Earth and Planetary Sciences(all)
Management of Technology and Innovation

Cite this

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title = "Correlation of partition coefficients of enzymes and amino acids in dextran+poly (ethylene glycol)+water aqueous two-phase systems",

abstract = "The partition coefficients of hydrolytic enzymes (α-amylase, β-amylase and glucoamylase) and amino acids (glycine, phenylalanine and isoleucine) in dextran+poly (ethylene glycol)+water aqueous two-phase systems were correlated by using the osmotic virial equation, the conventional Flory-Huggins equation, and a modified Flory-Huggins equation which were empirically proposed for aqueous polymer solutions, and their correlation performance are presented. Moreover, the effects of the molecular weights and the concentrations of polymers on the partition coefficients were also discussed. The interaction parameters required were determined by fitting the equations to the experimental partition coefficient data and good agreement was obtained.",

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AU - Zhou, Fan

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AU - Raghoe, Satien Dew

AU - Kakisaka, Keijiro

AU - Furuya, Takeshi

AU - Iwai, Yoshio

AU - Arai, Yasuhiko

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AB - The partition coefficients of hydrolytic enzymes (α-amylase, β-amylase and glucoamylase) and amino acids (glycine, phenylalanine and isoleucine) in dextran+poly (ethylene glycol)+water aqueous two-phase systems were correlated by using the osmotic virial equation, the conventional Flory-Huggins equation, and a modified Flory-Huggins equation which were empirically proposed for aqueous polymer solutions, and their correlation performance are presented. Moreover, the effects of the molecular weights and the concentrations of polymers on the partition coefficients were also discussed. The interaction parameters required were determined by fitting the equations to the experimental partition coefficient data and good agreement was obtained.

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Correlation of partition coefficients of enzymes and amino acids in dextran+poly (ethylene glycol)+water aqueous two-phase systems

Abstract

All Science Journal Classification (ASJC) codes

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