Correlation of telomerase activity with development and progression of adult t-cell leukemia

N. Uchida, T. Olsuka, F. Arima, H. Shigematsu, T. Fukuyama, M. Maeda, S. Suglo, Y. Itoh, Y. Niho

Research output: Contribution to journalArticlepeer-review

Abstract

Telomerase is an enzyme that adds hexametric TTAGGG nucleotide repeats to the ends of vertebrate chromosomal DMAs (i.e., telomeres) to compensate for losses that occur with each round of DNA replication. Telomerase activity, demonstrable in most human tumors, enables them to maintain telomere stability. Peripheral blood mononuclear cells were sampled from 57 patients seropositive for human Tlymphotropic virus type I (HTLV-I), including many with adult T-cell leukemia (ATL). Telomerase activity was determined in samples using a modified telomeric repeat amplification protocol. We semiquantitatively determined telomerase activity by serial dilution of each sample. All samples from acute and chronic type ATL patients were positive, 7 of 10 (70%) smoldering type patients and 7 of 24 (29.2%) asymptomatic viral carriers were positive. Disease progression from asymptomatic viral carrier to acute type correlated with telomerase activity. Two samples from chronic type ATL patients with relatively high telomerase activity progressed to the acute type within 1 month. Serum lactate dehydrogenase level also correlated with telomerase activity. These results indicate that reactivation of telomerase activity is a key event in development and progression of ATL, and telomerase could be a useful marker for predicting the course.

Original languageEnglish
Number of pages1
JournalExperimental Hematology
Volume26
Issue number8
Publication statusPublished - Dec 1 1998

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'Correlation of telomerase activity with development and progression of adult t-cell leukemia'. Together they form a unique fingerprint.

Cite this