Correlations between thymidylate synthase expression and chemosensitivity to 5-fluorouracil, cell proliferation and clinical outcome in head and neck squamous cell carcinoma

Ryuji Yasumatsu, Torahiko Nakashima, Hideoki Uryu, Toranoshin Ayada, Takahiro Wakasaki, Ryunosuke Kogo, Muneyuki Masuda, Masakazu Fukushima, Shizuo Komune

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: 5-Fluorouracil (5-FU) is a widely used drug in head and neck squamous cell carcinoma (HNSCC). Thymidylate synthase (TS), which is the target enzyme of 5-FU, has been demonstrated to be a key regulatory enzyme. In this study, we examined whether TS expression is correlated with chemosensitivity to 5-FU, cell proliferation and clinical outcome in HNSCC. Methods: An antisense TS cDNA was constitutively expressed in the HNSCC cell line. The effects of TS expression on in vitro cell growth and 5-FU cytotoxicity were examined. We also evaluated the association between TS expression and cell proliferation in surgical specimens, and prognosis in HNSCC patients. Results: Antisense TS transfection increases the cytotoxicity of 5-FU and inhibits cell proliferation in HNSCC cellsin vitro. Immunohistochemical expression of TS may have prognostic value in patients with HNSCC. Conclusions: These results indicate that TS expression plays an important role in the sensitivity of HNSCC to 5-FU chemotherapy.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalCHEMOTHERAPY
Volume55
Issue number1
DOIs
Publication statusPublished - Dec 2008

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pharmacology
  • Drug Discovery
  • Pharmacology (medical)
  • Infectious Diseases

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