Crassulacean acid metabolism may alleviate production of reactive oxygen species in a facultative CAM plant, the common ice plant Mesembryanthemum Crystallinum L.

Haruki Sunagawa, John C. Cushman, Sakae Agarie

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined the function of CAM in reactive oxygen species (ROS) alleviation using a newly isolated CAM-defi cient mutant of a facultative halophyte Mesembryanthemum crystallinum L. Salt-stress (0.4 M NaCl) induced nocturnal malate synthesis in the leaves of the wild-type plant, but not in the mutant. The content of hydrogen peroxide (H2O2) increased with elapse of time under salt-stress, but it dropped accompanied by the expression of CAM in the wild-type plants. The CAM-performing wild-type plant grown with 0.4 M NaCl for 12 d showed signifi cantly larger diel fl uctuation of malate and signifi cantly lower levels of H2O2 than the mutant, particularly at the end of the light period. The transcript abundance of a gene encoding plastidic Cu/Zn-superoxide dismutase (SOD), a marker of ROS production, was higher in the leaves of mutant plants than in those of wild-type plants. These results indicated that the performance of CAM was accompanied by lower levels of ROS and that CAM may help to alleviate oxidative stress under conditions of environmental stress.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
JournalPlant Production Science
Volume13
Issue number3
DOIs
Publication statusPublished - Jul 16 2010
Externally publishedYes

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Mesembryanthemum crystallinum
Crassulacean acid metabolism
reactive oxygen species
mutants
malates
salt stress
photophase
hydrogen peroxide
leaves
superoxide dismutase
oxidative stress
environmental factors
synthesis
genes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Crassulacean acid metabolism may alleviate production of reactive oxygen species in a facultative CAM plant, the common ice plant Mesembryanthemum Crystallinum L. / Sunagawa, Haruki; Cushman, John C.; Agarie, Sakae.

In: Plant Production Science, Vol. 13, No. 3, 16.07.2010, p. 256-260.

Research output: Contribution to journalArticle

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