Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death

Shoji Notomi, Toshio Hisatomi, Takaaki Kanemaru, Atsunobu Takeda, Yasuhiro Ikeda, Hiroshi Enaida, Guido Kroemer, Tatsuro Ishibashi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Stressed cells release ATP, which participates in neurodegenerative processes through the specific ligation of P2RX7 purinergic receptors. Here, we demonstrate that extracellular ATP and the more specific P2RX7 agonist, 2′- and 3′-O-(4-benzoylbenzoyl)-ATP, both induce photoreceptor cell death when added to primary retinal cell cultures or when injected into the eyes from wild-type mice, but not into the eyes from P2RX7 -/- mice. Photoreceptor cell death was accompanied by the activation of caspase-8 and -9, translocation of apoptosis-inducing factor from mitochondria to nuclei, and TUNEL-detectable chromatin fragmentation. All hallmarks of photoreceptor apoptosis were prevented by premedication or co-application of Brilliant Blue G, a selective P2RX7 antagonist that is already approved for the staining of internal limiting membranes during ocular surgery. ATP release is up-regulated by nutrient starvation in primary retinal cell cultures and seems to be an initializing event that triggers primary and/or secondary cell death via the positive feedback loop on P2RX7. Our results encourage the potential application of Brilliant Blue G as a novel neuroprotective agent in retinal diseases or similar neurodegenerative pathologies linked to excessive extracellular ATP.

Original languageEnglish
Pages (from-to)2798-2809
Number of pages12
JournalAmerican Journal of Pathology
Volume179
Issue number6
DOIs
Publication statusPublished - Dec 1 2011

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Purinergic Receptors
Photoreceptor Cells
Cell Death
Adenosine Triphosphate
Primary Cell Culture
Apoptosis Inducing Factor
Retinal Diseases
Premedication
Caspase 9
Caspase 8
In Situ Nick-End Labeling
Neuroprotective Agents
Starvation
Chromatin
Ligation
Mitochondria
Apoptosis
Pathology
Staining and Labeling
Food

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. / Notomi, Shoji; Hisatomi, Toshio; Kanemaru, Takaaki; Takeda, Atsunobu; Ikeda, Yasuhiro; Enaida, Hiroshi; Kroemer, Guido; Ishibashi, Tatsuro.

In: American Journal of Pathology, Vol. 179, No. 6, 01.12.2011, p. 2798-2809.

Research output: Contribution to journalArticle

Notomi, Shoji ; Hisatomi, Toshio ; Kanemaru, Takaaki ; Takeda, Atsunobu ; Ikeda, Yasuhiro ; Enaida, Hiroshi ; Kroemer, Guido ; Ishibashi, Tatsuro. / Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. In: American Journal of Pathology. 2011 ; Vol. 179, No. 6. pp. 2798-2809.
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