Critical role of cortactin in actin ring formation and osteoclastic bone resorption

Takuma Matsubara, Akira Myoui, Fumiyo Ikeda, Kenji Hata, Hideki Yoshikawa, Riko Nishimura, Toshiyuki Yoneda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Tyrosine kinase c-Src plays an essential role in ruffled border formation and bone resorption in osteoclasts; however, it is unclear how c-Src controls ruffled border formation during bone resorption. To address this question, we investigated the role of cortactin, a c-Src substrate, in osteoclasts. We found that cortactin showed colocalization with c-Src and actin rings in osteoclasts. Overexpression of cortactin stimulated actin ring formation in RAW 264.7 cells. In contrast, overexpression of Csk inhibited tyrosine phosphorylation of cortactin and binding of cortactin to c-Src. More importantly, overexertion of a mutant cortactin strongly suppressed actin ring formation and bone resorbing activity in osteoclasts. Collectively, our data indicate that cortactin controls osteoclastic bone resorption by regulating actin organization.

Original languageEnglish
Pages (from-to)368-372
Number of pages5
JournalJournal of Bone and Mineral Metabolism
Volume24
Issue number5
DOIs
Publication statusPublished - Sep 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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