Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling

Minako Ishibashi, Ken Ichi Hiasa, Qingwei Zhao, Shujiro Inoue, Kisho Ohtani, Shiro Kitamoto, Miyuki Tsuchihashi, Takeshi Sugaya, Israel F. Charo, Shinobu Kura, Teruhisa Tsuzuki, Tatsuro Ishibashi, Akira Takeshita, Kensuke Egashira

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Activated monocytes are present in the arterial walls of hypertensive patients and animals. Monocyte chemoattractant protein-1 (MCP-1), which controls monocyte function through its receptor (CCR2), is implicated in hypertensive inflammatory changes in the arterial wall. The role of CCR2 expression on monocytes in hypertension-induced vascular remodeling, however, has not been addressed. We hypothesized that CCR2 on monocytes is critical in hypertension-induced vascular inflammation and remodeling. Hypertension was induced by infusion of angiotensin II (Ang II) into wild-type mice, CCR2-deficient (CCR2-/-) mice, and bone marrow-transferred mice with a leukocyte-selective CCR2 deficiency (BMT-CCR2-/-). In wild-type mice, Ang II increased CCR2 intensity in circulating monocytes, which was prevented by an Ang II type-1 (AT1) receptor blocker or blunted in AT1 receptor-deficient mice. Enhanced CCR2 intensity on monocytes was observed in hypertensive patients and rats, and was reduced by treatment with the Ang II receptor blocker, supporting the clinical relevance of the observation in mice. In CCR2-/- and BMT-CCR2-/- mice, Ang II-induced vascular inflammation and vascular remodeling (aortic wall thickening and fibrosis) were blunted as compared with control mice. In contrast, Ang II-induced left ventricular hypertrophy developed in CCR2 -/- and BMT-CCR2-/- mice. The present study suggests that CCR2 expression in monocytes has a critical role in vascular inflammation and remodeling in Ang II-induced hypertension, and possibly in other forms of hypertension.

Original languageEnglish
Pages (from-to)1203-1210
Number of pages8
JournalCirculation research
Volume94
Issue number9
DOIs
Publication statusPublished - May 14 2004

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CCR2 Receptors
Monocytes
Hypertension
Inflammation
Angiotensin II
Angiotensin II Type 1 Receptor Blockers
Vascular Remodeling
Chemokine CCL2
Angiotensin Receptor Antagonists
Left Ventricular Hypertrophy
Blood Vessels
Leukocytes
Fibrosis
Bone Marrow
Observation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling. / Ishibashi, Minako; Hiasa, Ken Ichi; Zhao, Qingwei; Inoue, Shujiro; Ohtani, Kisho; Kitamoto, Shiro; Tsuchihashi, Miyuki; Sugaya, Takeshi; Charo, Israel F.; Kura, Shinobu; Tsuzuki, Teruhisa; Ishibashi, Tatsuro; Takeshita, Akira; Egashira, Kensuke.

In: Circulation research, Vol. 94, No. 9, 14.05.2004, p. 1203-1210.

Research output: Contribution to journalArticle

Ishibashi, M, Hiasa, KI, Zhao, Q, Inoue, S, Ohtani, K, Kitamoto, S, Tsuchihashi, M, Sugaya, T, Charo, IF, Kura, S, Tsuzuki, T, Ishibashi, T, Takeshita, A & Egashira, K 2004, 'Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling', Circulation research, vol. 94, no. 9, pp. 1203-1210. https://doi.org/10.1161/01.RES.0000126924.23467.A3
Ishibashi, Minako ; Hiasa, Ken Ichi ; Zhao, Qingwei ; Inoue, Shujiro ; Ohtani, Kisho ; Kitamoto, Shiro ; Tsuchihashi, Miyuki ; Sugaya, Takeshi ; Charo, Israel F. ; Kura, Shinobu ; Tsuzuki, Teruhisa ; Ishibashi, Tatsuro ; Takeshita, Akira ; Egashira, Kensuke. / Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling. In: Circulation research. 2004 ; Vol. 94, No. 9. pp. 1203-1210.
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AU - Ishibashi, Minako

AU - Hiasa, Ken Ichi

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AU - Inoue, Shujiro

AU - Ohtani, Kisho

AU - Kitamoto, Shiro

AU - Tsuchihashi, Miyuki

AU - Sugaya, Takeshi

AU - Charo, Israel F.

AU - Kura, Shinobu

AU - Tsuzuki, Teruhisa

AU - Ishibashi, Tatsuro

AU - Takeshita, Akira

AU - Egashira, Kensuke

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N2 - Activated monocytes are present in the arterial walls of hypertensive patients and animals. Monocyte chemoattractant protein-1 (MCP-1), which controls monocyte function through its receptor (CCR2), is implicated in hypertensive inflammatory changes in the arterial wall. The role of CCR2 expression on monocytes in hypertension-induced vascular remodeling, however, has not been addressed. We hypothesized that CCR2 on monocytes is critical in hypertension-induced vascular inflammation and remodeling. Hypertension was induced by infusion of angiotensin II (Ang II) into wild-type mice, CCR2-deficient (CCR2-/-) mice, and bone marrow-transferred mice with a leukocyte-selective CCR2 deficiency (BMT-CCR2-/-). In wild-type mice, Ang II increased CCR2 intensity in circulating monocytes, which was prevented by an Ang II type-1 (AT1) receptor blocker or blunted in AT1 receptor-deficient mice. Enhanced CCR2 intensity on monocytes was observed in hypertensive patients and rats, and was reduced by treatment with the Ang II receptor blocker, supporting the clinical relevance of the observation in mice. In CCR2-/- and BMT-CCR2-/- mice, Ang II-induced vascular inflammation and vascular remodeling (aortic wall thickening and fibrosis) were blunted as compared with control mice. In contrast, Ang II-induced left ventricular hypertrophy developed in CCR2 -/- and BMT-CCR2-/- mice. The present study suggests that CCR2 expression in monocytes has a critical role in vascular inflammation and remodeling in Ang II-induced hypertension, and possibly in other forms of hypertension.

AB - Activated monocytes are present in the arterial walls of hypertensive patients and animals. Monocyte chemoattractant protein-1 (MCP-1), which controls monocyte function through its receptor (CCR2), is implicated in hypertensive inflammatory changes in the arterial wall. The role of CCR2 expression on monocytes in hypertension-induced vascular remodeling, however, has not been addressed. We hypothesized that CCR2 on monocytes is critical in hypertension-induced vascular inflammation and remodeling. Hypertension was induced by infusion of angiotensin II (Ang II) into wild-type mice, CCR2-deficient (CCR2-/-) mice, and bone marrow-transferred mice with a leukocyte-selective CCR2 deficiency (BMT-CCR2-/-). In wild-type mice, Ang II increased CCR2 intensity in circulating monocytes, which was prevented by an Ang II type-1 (AT1) receptor blocker or blunted in AT1 receptor-deficient mice. Enhanced CCR2 intensity on monocytes was observed in hypertensive patients and rats, and was reduced by treatment with the Ang II receptor blocker, supporting the clinical relevance of the observation in mice. In CCR2-/- and BMT-CCR2-/- mice, Ang II-induced vascular inflammation and vascular remodeling (aortic wall thickening and fibrosis) were blunted as compared with control mice. In contrast, Ang II-induced left ventricular hypertrophy developed in CCR2 -/- and BMT-CCR2-/- mice. The present study suggests that CCR2 expression in monocytes has a critical role in vascular inflammation and remodeling in Ang II-induced hypertension, and possibly in other forms of hypertension.

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