Crocin suppresses tumor necrosis factor-α-induced cell death of neuronally differentiated PC-12 cells

Shinji Soeda, Takashi Ochiai, Loungratana Paopong, Hiroyuki Tanaka, Yukihiro Shoyama, Hiroshi Shimeno

Research output: Contribution to journalArticlepeer-review

137 Citations (Scopus)

Abstract

Crocus sativus L. is used in Chinese traditional medicine to treat some disorders of the central nervous system. Crocin is an ethanol-extractable component of Crocus sativus L.; it is reported to prevent ethanol-induced impairment of learning and memory in mice. In this study, we demonstrate that crocin suppresses the effect of tumor necrosis factor (TNF)-α on neuronally differentiated PC-12 cells. PC-12 cells dead from exposure to TNF-α show apoptotic morphological changes and DNA fragmentation. These hallmark features of cell death did not appear in cells treated in the co-presence of 10 μM crocin. Moreover, crocin suppressed the TNF-α-induced expression of Bcl-XS and LICE mRNAs and simultaneously restored the cytokine-induced reduction of Bcl-XL mRNA expression. The modulating effects of crocin on the expression of Bcl-2 family proteins led to a marked reduction of a TNF-α-induced release of cytochrome c from the mitochondria. Crocin also blocked the cytochrome c-induced activation of caspase-3. To learn how crocin exhibits these anti-apoptotic actions in PC-12 cells, we tested the effect of crocin on PC-12 cell death induced by daunorubicin. We found that crocin inhibited the effect of daunorubicin as well. Our findings suggest that crocin inhibits neuronal cell death induced by both internal and external apoptotic stimuli.

Original languageEnglish
Pages (from-to)2887-2898
Number of pages12
JournalLife Sciences
Volume69
Issue number24
DOIs
Publication statusPublished - Nov 2 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Crocin suppresses tumor necrosis factor-α-induced cell death of neuronally differentiated PC-12 cells'. Together they form a unique fingerprint.

Cite this