Cross-linking strategy for molecular recognition and fluorescent sensing of a multi-phosphorylated peptide in aqueous solution

Akio Ojida, Masa Aki Inoue, Yasuko Mito-oka, Itaru Hamachi

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

In the research field of molecular recognition, selective recognition and sensing of phosphorylated protein surfaces is strongly desirable both for elucidation of protein-protein recognition at the molecular level and for regulation of signal transduction through protein surfaces. Here we describe a new strategy for molecular recognition of a multi-phosphorylated peptide using intrapeptide cross-linking on the basis of coordination chemistry. The present artificial receptor can selectively bind to doubly phosphorylated peptide through multiple-point interactions and fluorescently sense the binding event with an association constant of more than 106 M-1 in neutral aqueous solution.

Original languageEnglish
Pages (from-to)10184-10185
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number34
DOIs
Publication statusPublished - Aug 27 2003

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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