Cross-linking versus non-cross-linking aggregation of gold nanoparticles induced by DNA hybridization: A comparison of the rapidity of solution color change

Guoqing Wang, Yoshitsugu Akiyama, Shota Shiraishi, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Gold nanoparticles densely modified with single-stranded DNA (ssDNA-AuNPs) form aggregates with cross-linker ssDNAs via duplex formation. Alternatively, the ssDNA-AuNPs are spontaneously aggregated at high ionic strength in a non-cross-linking manner when complementary ssDNAs are added to form fully matched duplexes. Both aggregation modes are accompanied by a red-to-purple color change, which has been exploited in various bioassays. The current study compares the rapidity of color change between the cross-linking and non-cross-linking aggregation modes under identical conditions. When a small number of cross-linker/complementary DNAs are provided, the cross-linking mode exhibited more rapid color change than the non-cross-linking mode. Conversely, with a large number of the DNAs, the non-cross-linking aggregation occurred more rapidly than the cross-linking counterpart. This finding allows one to select a more appropriate aggregation mode for application of ssDNA-AuNPs to colorimetric assays under given conditions.

Original languageEnglish
Pages (from-to)270-277
Number of pages8
JournalBioconjugate Chemistry
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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