Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid

K. Watanabe, M. Matsuda, Y. Tateoka, T. Kimura, T. Matsunaga, H. Tanaka, Y. Shoyama, I. Yamamoto

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A monoclonal antibody (MAb-4A4) against Δ9-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Δ9-THC (1-100 μg/ml) could be determined by the ELISA. Many metabolites of Δ8-THC and Δ9-THC reacted with the antibody, and their cross-reactivities were 44-157% of Δ8-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, β-carotene, androstene-3,17-dione or an endogenous cannabinoid, anandamide.

Original languageEnglish
Pages (from-to)310-313
Number of pages4
JournalJournal of Health Science
Volume46
Issue number4
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

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