TY - JOUR
T1 - Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid
AU - Watanabe, K.
AU - Matsuda, M.
AU - Tateoka, Y.
AU - Kimura, T.
AU - Matsunaga, T.
AU - Tanaka, H.
AU - Shoyama, Y.
AU - Yamamoto, I.
PY - 2000
Y1 - 2000
N2 - A monoclonal antibody (MAb-4A4) against Δ9-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Δ9-THC (1-100 μg/ml) could be determined by the ELISA. Many metabolites of Δ8-THC and Δ9-THC reacted with the antibody, and their cross-reactivities were 44-157% of Δ8-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, β-carotene, androstene-3,17-dione or an endogenous cannabinoid, anandamide.
AB - A monoclonal antibody (MAb-4A4) against Δ9-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Δ9-THC (1-100 μg/ml) could be determined by the ELISA. Many metabolites of Δ8-THC and Δ9-THC reacted with the antibody, and their cross-reactivities were 44-157% of Δ8-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, β-carotene, androstene-3,17-dione or an endogenous cannabinoid, anandamide.
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U2 - 10.1248/jhs.46.310
DO - 10.1248/jhs.46.310
M3 - Article
AN - SCOPUS:0033863941
SN - 1344-9702
VL - 46
SP - 310
EP - 313
JO - Journal of Health Science
JF - Journal of Health Science
IS - 4
ER -