TY - GEN
T1 - Crosstalk between giant DNA and actin filament in a model cellular system
AU - Negishi, Makiko
AU - Sakaue, Takahiro
AU - Takiguchi, Kingo
AU - Yoshikawa, Kenichi
PY - 2009/12/1
Y1 - 2009/12/1
N2 - We confined actin and giant DNA mixture in a phospholipid coated micrometer scale (cell size) closed space (PMS) as a cell nuclear model system. We report the appearance of a spatially segregated state in PMSs between actin filaments and giant DNA molecules. When the diameter of a PMS was below 15 μm, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. Furthermore, when the coexisting actin concentration was high enough, the aggregation of actin and DNA were still observed on the surface of a PMS whose diameter was above 15 μm. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpreted these results in terms of the generation of a depletion zone near a surface with actin filaments in a PMS.
AB - We confined actin and giant DNA mixture in a phospholipid coated micrometer scale (cell size) closed space (PMS) as a cell nuclear model system. We report the appearance of a spatially segregated state in PMSs between actin filaments and giant DNA molecules. When the diameter of a PMS was below 15 μm, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. Furthermore, when the coexisting actin concentration was high enough, the aggregation of actin and DNA were still observed on the surface of a PMS whose diameter was above 15 μm. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpreted these results in terms of the generation of a depletion zone near a surface with actin filaments in a PMS.
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U2 - 10.1109/MHS.2009.5352054
DO - 10.1109/MHS.2009.5352054
M3 - Conference contribution
AN - SCOPUS:77950954468
SN - 9781424450954
T3 - 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
SP - 150
EP - 154
BT - 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
T2 - 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
Y2 - 8 November 2009 through 11 November 2009
ER -