Crosstalk between giant DNA and actin filament in a model cellular system

Makiko Negishi, Takahiro Sakaue, Kingo Takiguchi, Kenichi Yoshikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We confined actin and giant DNA mixture in a phospholipid coated micrometer scale (cell size) closed space (PMS) as a cell nuclear model system. We report the appearance of a spatially segregated state in PMSs between actin filaments and giant DNA molecules. When the diameter of a PMS was below 15 μm, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. Furthermore, when the coexisting actin concentration was high enough, the aggregation of actin and DNA were still observed on the surface of a PMS whose diameter was above 15 μm. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpreted these results in terms of the generation of a depletion zone near a surface with actin filaments in a PMS.

Original languageEnglish
Title of host publication20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
Pages150-154
Number of pages5
DOIs
Publication statusPublished - Dec 1 2009
Event20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science - Nagoya, Japan
Duration: Nov 8 2009Nov 11 2009

Publication series

Name20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science

Other

Other20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
CountryJapan
CityNagoya
Period11/8/0911/11/09

Fingerprint

Crosstalk
DNA
Molecules
Phospholipids
Agglomeration

All Science Journal Classification (ASJC) codes

  • Computer Science(all)
  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Negishi, M., Sakaue, T., Takiguchi, K., & Yoshikawa, K. (2009). Crosstalk between giant DNA and actin filament in a model cellular system. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science (pp. 150-154). [5352054] (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science). https://doi.org/10.1109/MHS.2009.5352054

Crosstalk between giant DNA and actin filament in a model cellular system. / Negishi, Makiko; Sakaue, Takahiro; Takiguchi, Kingo; Yoshikawa, Kenichi.

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 150-154 5352054 (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Negishi, M, Sakaue, T, Takiguchi, K & Yoshikawa, K 2009, Crosstalk between giant DNA and actin filament in a model cellular system. in 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science., 5352054, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, pp. 150-154, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, Nagoya, Japan, 11/8/09. https://doi.org/10.1109/MHS.2009.5352054
Negishi M, Sakaue T, Takiguchi K, Yoshikawa K. Crosstalk between giant DNA and actin filament in a model cellular system. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 150-154. 5352054. (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science). https://doi.org/10.1109/MHS.2009.5352054
Negishi, Makiko ; Sakaue, Takahiro ; Takiguchi, Kingo ; Yoshikawa, Kenichi. / Crosstalk between giant DNA and actin filament in a model cellular system. 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. pp. 150-154 (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science).
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