TY - JOUR
T1 - Crosstalk of hedgehog and Wnt pathways in gastric cancer
AU - Yanai, Kosuke
AU - Nakamura, Masafumi
AU - Akiyoshi, Takashi
AU - Nagai, Shuntaro
AU - Wada, Junji
AU - Koga, Kenichiro
AU - Noshiro, Hirokazu
AU - Nagai, Eishi
AU - Tsuneyoshi, Masazumi
AU - Tanaka, Masao
AU - Katano, Mitsuo
N1 - Funding Information:
This study was supported by a General Scientific Research Grant (18659372, 18390350) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Dr. Akihide Ryo (Yokohama City University School of Medicine, Yokohama, Japan) and Dr. Hiroshi Sasaki (Riken Center for Developmental Biology, Kobe, Japan) for plasmids; Dr. Koji Hokazono (Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan) for help with experiments; and Kaori Nomiyama, Nobuhiro Torata, and Miyuki Manabe for skilful technical assistance.
PY - 2008/5/8
Y1 - 2008/5/8
N2 - Morphogenic signals like Hedgehog (Hh) and Wnt are reported to play critical roles in the progression of gastric cancer. We aimed to assess the relationship between Hh and Wnt signaling pathways. In 58 gastric cancer specimens, Wnt pathway activation was inversely correlated with Hh pathway activation. When AGS gastric cancer cells, in which Wnt signaling was constitutively active, were used as a target cell line, Gli1 overexpression suppressed Wnt transcriptional activity, nuclear β-catenin accumulation and proliferation of AGS cells. Knock-down of β-catenin by siRNA suppressed Wnt pathway activity and proliferation of AGS cells. Our data may provide some clues for the treatment of gastric cancer associated with Wnt signaling activation.
AB - Morphogenic signals like Hedgehog (Hh) and Wnt are reported to play critical roles in the progression of gastric cancer. We aimed to assess the relationship between Hh and Wnt signaling pathways. In 58 gastric cancer specimens, Wnt pathway activation was inversely correlated with Hh pathway activation. When AGS gastric cancer cells, in which Wnt signaling was constitutively active, were used as a target cell line, Gli1 overexpression suppressed Wnt transcriptional activity, nuclear β-catenin accumulation and proliferation of AGS cells. Knock-down of β-catenin by siRNA suppressed Wnt pathway activity and proliferation of AGS cells. Our data may provide some clues for the treatment of gastric cancer associated with Wnt signaling activation.
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U2 - 10.1016/j.canlet.2007.12.030
DO - 10.1016/j.canlet.2007.12.030
M3 - Article
C2 - 18243529
AN - SCOPUS:41049117410
VL - 263
SP - 145
EP - 156
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -
