Cryogel formation by cellulose sulfate

In order to identify the role of heparin in the process of cryogel formation, variously sulfonated cellulose (SC) were prepared and their gel forming abilities were evaluated by the measurement of temperature dependence of turbidity in solution mixed with fibronectin, fibrinogen and SC. We found that the SC which sulfonated 100% of 6 position and 36% of 2 position in glucose residue in cellulose displayed excellent performances to form gel. Gel forming profiles of this SG also just fitted with that of heparin. These results is to be expected that the most important chemical structure of heparin for gel forming is the 2nd position in glucose residue.