Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy

Yasuhiro Utsumi, Yuzou Sano

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.

Original languageEnglish
Title of host publicationElectron Microscopy
Subtitle of host publicationMethods and Protocols
EditorsJohn Kuo
Pages497-506
Number of pages10
DOIs
Publication statusPublished - Feb 27 2007

Publication series

NameMethods in Molecular Biology
Volume369
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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