TY - JOUR
T1 - Cryptic diversity in the widespread Asian ant Crematogaster rothneyi (Hymenoptera
T2 - Formicidae) inferred from morphological and genetic evidence
AU - Hosoishi, Shingo
AU - Ogata, Kazuo
N1 - Funding Information:
This work and the new species name have been registered with ZooBank under urn:lsid:zoobank.org:pub:A17C36B5-0A85-4CB3- A88D-47CC7EE7659. We would like to thank Dr. Seiki Yamane for providing specimens. We would also like to thank Mr. Phourin Chhang and Mr. Vanna Samreth (Forestry Administration, Phnom Penh, Cambodia), Dr. Tsuyoshi Kajisa (Kagoshima University) and Dr. Nobuya Mizoue (Faculty of Agriculture, Kyushu University) and Dr. Tetsukazu Yahara (Faculty of Science, Kyushu University) for helping with our field surveys in this study. We are grateful to the following persons for loaning us material or allowing us access to museum collections: Bernhard Merz (MHNG) and Dominique Zimmermann (NHMW). We would like to thank ANeT members for their encouragement. Thanks are also due to Mark Lorenz (Forte Inc.) for improving the English of this manuscript. This work was supported in part by JSPS KAKENHI (Grant-in-Aid for Scientific Research (C)) Grant Number 26440221, JSPS KAKENHI (Grant-in-Aid for Scientific Research (B)) Grant Number 26304014 and Global COE Program (Center of excellence for Asian conservation ecology as a basis of human-nature mutualism), MEXT, Japan. Thanks also to the three anonymous reviewers for their constructive improvements to the manuscript.
Funding Information:
name have been registered with ZooBank under urn:lsid:zoobank.org:pub:A17C36B5-0A85-4CB3-A88D-47CC7EE7659. We would like to thank Dr. Seiki Yamane for providing specimens. We would also like to thank Mr. Phourin Chhang and Mr. Vanna Samreth (Forestry Administration, Phnom Penh, Cambodia), Dr. Tsuyoshi Kajisa (Kagoshima University) and Dr. Nobuya Mizoue (Faculty of Agriculture, Kyushu University) and Dr. Tetsukazu Yahara (Faculty of Science, Kyushu University) for helping with our field surveys in this study. We are grateful to the following persons for loaning us material or allowing us access to museum collections: Bernhard Merz (MHNG) and Dominique Zimmermann (NHMW). We would like to thank ANeT members for their encouragement. Thanks are also due to Mark Lorenz (Forte Inc.) for improving the English of this manuscript. This work was supported in part by JSPS KAKENHI (Grant-in-Aid for Scientific Research (C)) Grant Number 26440221, JSPS KAKENHI (Grant-in-Aid for Scientific Research (B)) Grant Number 26304014 and Global COE Program
Publisher Copyright:
© 2019 Academia Sinica, Taiwan.
PY - 2019
Y1 - 2019
N2 - The myrmicine species Crematogaster rothneyi is one of the most widely distributed ants in Asia, but it has rarely been collected in the field. Its distribution range covers South and Southeast Asia, extending approximately 5,000 km from India in the west to Sulawesi in the east. Despite this wide distribution range, C. rothneyi has been treated as a single taxonomic species, and no combined morphological or molecular analysis has been conducted to assess whether any intraspecific variation exists. The sequence divergences of C. rothneyi populations, mainly obtained from Southeast Asia, were investigated by analyzing 387 bp and 175 bp sequences of the 12S ribosomal RNA and cytochrome c oxidase subunit (COI) genes, respectively. Phylogenetic analysis indicated that the C. rothneyi populations were separated into three groups: Group I from Thailand and Cambodia, group II from Bangladesh and Myanmar, and group III from Krakatau and Sulawesi. Groups II and III were recovered as a single clade, sister to group I. The interspecific divergences were 7.3% to 8.5% for 12S and 14.5% to 23.3% for COI between most C. rothneyi specimens and Cambodian specimens, while divergence for 12S was 3.5% between Thai and Cambodian specimens. The Thai specimens are not morphologically differentiated, and are considered conspecific to C. rothneyi. We describe the Cambodian series as a new species, Crematogaster yaharai sp. nov., based on unique antennal morphology and mesosomal sculpture patterns with molecular evidence. Crematogaster rothneyi civa Forel, 1902 is synonymized under C. rothneyi. Crematogaster rothneyi is widespread and has sister species, suggesting recent diversification within the Indochinese Peninsula.
AB - The myrmicine species Crematogaster rothneyi is one of the most widely distributed ants in Asia, but it has rarely been collected in the field. Its distribution range covers South and Southeast Asia, extending approximately 5,000 km from India in the west to Sulawesi in the east. Despite this wide distribution range, C. rothneyi has been treated as a single taxonomic species, and no combined morphological or molecular analysis has been conducted to assess whether any intraspecific variation exists. The sequence divergences of C. rothneyi populations, mainly obtained from Southeast Asia, were investigated by analyzing 387 bp and 175 bp sequences of the 12S ribosomal RNA and cytochrome c oxidase subunit (COI) genes, respectively. Phylogenetic analysis indicated that the C. rothneyi populations were separated into three groups: Group I from Thailand and Cambodia, group II from Bangladesh and Myanmar, and group III from Krakatau and Sulawesi. Groups II and III were recovered as a single clade, sister to group I. The interspecific divergences were 7.3% to 8.5% for 12S and 14.5% to 23.3% for COI between most C. rothneyi specimens and Cambodian specimens, while divergence for 12S was 3.5% between Thai and Cambodian specimens. The Thai specimens are not morphologically differentiated, and are considered conspecific to C. rothneyi. We describe the Cambodian series as a new species, Crematogaster yaharai sp. nov., based on unique antennal morphology and mesosomal sculpture patterns with molecular evidence. Crematogaster rothneyi civa Forel, 1902 is synonymized under C. rothneyi. Crematogaster rothneyi is widespread and has sister species, suggesting recent diversification within the Indochinese Peninsula.
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U2 - 10.6620/ZS.2019.58-11
DO - 10.6620/ZS.2019.58-11
M3 - Article
AN - SCOPUS:85075587283
VL - 58
JO - Zoological Studies
JF - Zoological Studies
SN - 1021-5506
M1 - 11
ER -