Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 Å resolution

Atsushi Nakagawa, Isao Tanaka, Ritsu Sakai, Takashi Nakashima, Gunki Funatsu, Makoto Kimura

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The ribonuclease MC1 (RNase MC1) from seeds of bitter gourd (Momordica charantia) consists of 190 amino acid residues with four disulfide bridges and belongs to the RNase T2 family, including fungal RNases typified by RNase Rh from Rhizopus niveus and RNase T2 from Aspergillus oryzae. The crystal structure of RNase MC1 has been determined at 1.75 A resolution with an R-factor of 19.7% using the single isomorphous replacement method. RNase MC1 structurally belongs to the (α+β) class of proteins, having ten helices (six α-helices and four 310-helices) and eight β-strands. When the structures of RNase MC1 and RNase Rh are superposed, the close agreement between the α-carbon positions for the total structure is obvious: the root mean square deviations calculated only for structurally related 151 α-carbon atoms of RNase MC1 and RNase Rh molecules was 1.76 A. Furthermore, the conformation of the catalytic residues His-46, Glu-105, and His-109 in RNase Rh can be easily superposed with that of the possible catalytic residues His-34, Glu-84, and His-88 in RNase MC1. This observation strongly indicates that RNase MC1 from a plant origin catalyzes RNA degradation in a similar manner as fungal RNases. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume1433
Issue number1-2
DOIs
Publication statusPublished - Aug 17 1999

Fingerprint

Momordica charantia
Ribonucleases
Seed
Seeds
Crystal structure
ribonuclease T(2)
R388
Carbon
Aspergillus oryzae
Rhizopus
Aspergillus
RNA Stability
ribonuclease MC1
Disulfides
Conformations
ribonuclease Rh
RNA
Amino Acids
Degradation
Atoms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 Å resolution. / Nakagawa, Atsushi; Tanaka, Isao; Sakai, Ritsu; Nakashima, Takashi; Funatsu, Gunki; Kimura, Makoto.

In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1433, No. 1-2, 17.08.1999, p. 253-260.

Research output: Contribution to journalArticle

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