Crystal Structure of HypA, a Nickel-Binding Metallochaperone for [NiFe] Hydrogenase Maturation

Satoshi Watanabe, Takayuki Arai, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

HypA is one of the auxiliary proteins involved in the maturation of [NiFe] hydrogenases. By an unknown mechanism, HypA functions as a metallochaperone in the insertion of the Ni atom into hydrogenases. We have determined the crystal structures of HypA from Thermococcus kodakaraensis KOD1 in both monomeric and dimeric states. The structure of the HypA monomer consists of Ni- and Zn-binding domains. The relative arrangement of the two metal-binding domains has been shown to be associated with local conformations of the conserved Ni-binding motif, suggesting a communication between the Ni- and Zn-binding sites. The HypA dimer has been shown to be stabilized by unexpected domain swapping through archaea-specific linker helices. In addition, the hexameric structure of HypA is formed in the crystal packing. Several hydrogen bonds and hydrophobic interactions stabilize the hexamer interface. These findings suggest the functional diversity of HypA proteins.

Original languageEnglish
Pages (from-to)448-459
Number of pages12
JournalJournal of Molecular Biology
Volume394
Issue number3
DOIs
Publication statusPublished - Dec 4 2009
Externally publishedYes

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Metallochaperones
Nickel
Thermococcus
Hydrogenase
Archaea
Hydrophobic and Hydrophilic Interactions
Hydrogen
Proteins
Metals
Binding Sites
nickel-iron hydrogenase

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Crystal Structure of HypA, a Nickel-Binding Metallochaperone for [NiFe] Hydrogenase Maturation. / Watanabe, Satoshi; Arai, Takayuki; Matsumi, Rie; Atomi, Haruyuki; Imanaka, Tadayuki; Miki, Kunio.

In: Journal of Molecular Biology, Vol. 394, No. 3, 04.12.2009, p. 448-459.

Research output: Contribution to journalArticle

Watanabe, Satoshi ; Arai, Takayuki ; Matsumi, Rie ; Atomi, Haruyuki ; Imanaka, Tadayuki ; Miki, Kunio. / Crystal Structure of HypA, a Nickel-Binding Metallochaperone for [NiFe] Hydrogenase Maturation. In: Journal of Molecular Biology. 2009 ; Vol. 394, No. 3. pp. 448-459.
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