Crystal structure of lignin peroxidase

Steven L. Edwards, Reetta Raag, Hiroyuki Wariishi, Michael H. Gold, Thomas L. Poulos

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The crystal structure of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium has been determined to 2.6 Å resolution by using multiple isomorphous replacement methods and simulated annealing refinement. Of the 343 residues, residues 3-335 have been accounted for in the electron density map, including four disulfide bonds. The overall three-dimensional structure is very similar to the only other peroxidase in this group for which a high-resolution crystal structure is available, cytochrome c peroxidase, despite the fact that the sequence identity is only ≈20%, LiP has four disulfide bonds, while cytochrome c peroxidase has none, and LiP is larger (343 vs. 294 residues). The basic helical fold and connectivity defined by 11 helical segments with the heme sandwiched between the distal and proximal helices found in cytochrome c peroxidase is maintained in LiP. Both enzymes have a histidine as a proximal heme ligand, which is hydrogen bonded to a buried aspartic acid side chain. The distal or peroxide binding pocket also is similar, including the distal arginine and histidine. The most striking difference is that, whereas cytochrome c peroxidase has tryptophans contacting the distal and proximal heme surfaces, LiP has phenylalanines. This in part explains why, in the reaction with peroxides, cytochrome c peroxidase forms an amino acid-centered free radical, whereas LiP forms a porphyrin π cation radical.

Original languageEnglish
Pages (from-to)750-754
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number2
DOIs
Publication statusPublished - Jan 15 1993

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Cytochrome-c Peroxidase
Heme
Peroxides
Histidine
Disulfides
Phanerochaete
Basidiomycota
Porphyrins
Phenylalanine
Aspartic Acid
Tryptophan
Peroxidase
Free Radicals
Arginine
lignin peroxidase
Cations
Hydrogen
Electrons
Ligands
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

Crystal structure of lignin peroxidase. / Edwards, Steven L.; Raag, Reetta; Wariishi, Hiroyuki; Gold, Michael H.; Poulos, Thomas L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 2, 15.01.1993, p. 750-754.

Research output: Contribution to journalArticle

Edwards, Steven L. ; Raag, Reetta ; Wariishi, Hiroyuki ; Gold, Michael H. ; Poulos, Thomas L. / Crystal structure of lignin peroxidase. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 2. pp. 750-754.
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