Crystal structure of rat heme oxygenase-1 in complex with heme

Masakazu Sugishima, Yoshiaki Omata, Yoshimitsu Kakuta, Hiroshi Sakamoto, Masato Noguchi, Keiichi Fukuyama

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Heme oxygenase catalyzes the oxidative cleavage of protoheme to biliverdin, the first step of heme metabolism utilizing O2 and NADPH. We determined the crystal structures of rat heme oxygenase-1 (HO-1)-heme and selenomethionyl HO-1-heme complexes. Heme is sandwiched between two helices with the δ-meso edge of the heme being exposed to the surface. Gly143N forms a hydrogen bond to the distal ligand of heme, OH-. The distance between Gly143N and the ligand is shorter than that in the human HO-1-heme complex. This difference may be related to a pH-dependent change of the distal ligand of heme. Flexibility of the distal helix may control the stability of the coordination of the distal ligand to heme iron. The possible role of Gly143 in the heme oxygenase reaction is discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalFEBS Letters
Volume471
Issue number1
DOIs
Publication statusPublished - Apr 7 2000
Externally publishedYes

Fingerprint

Heme Oxygenase-1
Heme
Rats
Crystal structure
Ligands
Heme Oxygenase (Decyclizing)
Biliverdine
NADP
Metabolism
Hydrogen
Hydrogen bonds
Iron

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Sugishima, M., Omata, Y., Kakuta, Y., Sakamoto, H., Noguchi, M., & Fukuyama, K. (2000). Crystal structure of rat heme oxygenase-1 in complex with heme. FEBS Letters, 471(1), 61-66. https://doi.org/10.1016/S0014-5793(00)01353-3

Crystal structure of rat heme oxygenase-1 in complex with heme. / Sugishima, Masakazu; Omata, Yoshiaki; Kakuta, Yoshimitsu; Sakamoto, Hiroshi; Noguchi, Masato; Fukuyama, Keiichi.

In: FEBS Letters, Vol. 471, No. 1, 07.04.2000, p. 61-66.

Research output: Contribution to journalArticle

Sugishima, M, Omata, Y, Kakuta, Y, Sakamoto, H, Noguchi, M & Fukuyama, K 2000, 'Crystal structure of rat heme oxygenase-1 in complex with heme', FEBS Letters, vol. 471, no. 1, pp. 61-66. https://doi.org/10.1016/S0014-5793(00)01353-3
Sugishima M, Omata Y, Kakuta Y, Sakamoto H, Noguchi M, Fukuyama K. Crystal structure of rat heme oxygenase-1 in complex with heme. FEBS Letters. 2000 Apr 7;471(1):61-66. https://doi.org/10.1016/S0014-5793(00)01353-3
Sugishima, Masakazu ; Omata, Yoshiaki ; Kakuta, Yoshimitsu ; Sakamoto, Hiroshi ; Noguchi, Masato ; Fukuyama, Keiichi. / Crystal structure of rat heme oxygenase-1 in complex with heme. In: FEBS Letters. 2000 ; Vol. 471, No. 1. pp. 61-66.
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