Crystal structure of the RuvA-RuvB complex: A structural basis for the holliday junction migrating motor machinery

Kazuhiro Yamada, Tomoko Miyata, Daisuke Tsuchiya, Takuji Oyama, Yoshie Fujiwara, Takayuki Ohnishi, Hiroshi Iwasaki, Hideo Shinagawa, Mariko Ariyoshi, Kouta Mayanagi, Kosuke Morikawa

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Abstract

We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding β hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.

Original languageEnglish
Pages (from-to)671-681
Number of pages11
JournalMolecular Cell
Volume10
Issue number3
DOIs
Publication statusPublished - Sep 1 2002

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Cruciform DNA
X-Rays
Adenosine Triphosphate
Electrons
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Yamada, K., Miyata, T., Tsuchiya, D., Oyama, T., Fujiwara, Y., Ohnishi, T., ... Morikawa, K. (2002). Crystal structure of the RuvA-RuvB complex: A structural basis for the holliday junction migrating motor machinery. Molecular Cell, 10(3), 671-681. https://doi.org/10.1016/S1097-2765(02)00641-X

Crystal structure of the RuvA-RuvB complex : A structural basis for the holliday junction migrating motor machinery. / Yamada, Kazuhiro; Miyata, Tomoko; Tsuchiya, Daisuke; Oyama, Takuji; Fujiwara, Yoshie; Ohnishi, Takayuki; Iwasaki, Hiroshi; Shinagawa, Hideo; Ariyoshi, Mariko; Mayanagi, Kouta; Morikawa, Kosuke.

In: Molecular Cell, Vol. 10, No. 3, 01.09.2002, p. 671-681.

Research output: Contribution to journalArticle

Yamada, K, Miyata, T, Tsuchiya, D, Oyama, T, Fujiwara, Y, Ohnishi, T, Iwasaki, H, Shinagawa, H, Ariyoshi, M, Mayanagi, K & Morikawa, K 2002, 'Crystal structure of the RuvA-RuvB complex: A structural basis for the holliday junction migrating motor machinery', Molecular Cell, vol. 10, no. 3, pp. 671-681. https://doi.org/10.1016/S1097-2765(02)00641-X
Yamada, Kazuhiro ; Miyata, Tomoko ; Tsuchiya, Daisuke ; Oyama, Takuji ; Fujiwara, Yoshie ; Ohnishi, Takayuki ; Iwasaki, Hiroshi ; Shinagawa, Hideo ; Ariyoshi, Mariko ; Mayanagi, Kouta ; Morikawa, Kosuke. / Crystal structure of the RuvA-RuvB complex : A structural basis for the holliday junction migrating motor machinery. In: Molecular Cell. 2002 ; Vol. 10, No. 3. pp. 671-681.
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