Crystal structures of the HypCD complex and the HypCDE ternary complex: Transient intermediate complexes during [NiFe] hydrogenase maturation

Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

[NiFe] hydrogenase maturation represents one of the most dynamic and sophisticated processes in metallocenter assembly. The Fe(CN)2CO moiety of [NiFe] hydrogenases is assembled via unknown transient interactions among specific maturation proteins HypC (metallochaperone), HypD (redox protein), and HypE (cyanide synthesis/donor). Here, we report the structures of the HypC-HypD and HypC-HypD-HypE complexes, providing a view of the transient interactions that take place during the maturation process. HypC binds to the conserved region of HypD through extensive hydrophobic interactions. The ternary complex formation between HypE and the HypCD complex involves both HypC and HypD, rendering the HypE conformation favorable for cyanide transfer. In the complex, the conserved cysteines of HypC and HypD form an Fe binding site. The conserved C-terminal cysteine of HypE can access the thiol redox cascade of HypD. These results provide structural insights into the Fe atom cyanation in the HypCDE complex.

Original languageEnglish
Pages (from-to)2124-2137
Number of pages14
JournalStructure
Volume20
Issue number12
DOIs
Publication statusPublished - Dec 5 2012
Externally publishedYes

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Cyanides
Oxidation-Reduction
Cysteine
Metallochaperones
Hydrophobic and Hydrophilic Interactions
Sulfhydryl Compounds
Proteins
Binding Sites
nickel-iron hydrogenase

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Structural Biology

Cite this

Crystal structures of the HypCD complex and the HypCDE ternary complex : Transient intermediate complexes during [NiFe] hydrogenase maturation. / Watanabe, Satoshi; Matsumi, Rie; Atomi, Haruyuki; Imanaka, Tadayuki; Miki, Kunio.

In: Structure, Vol. 20, No. 12, 05.12.2012, p. 2124-2137.

Research output: Contribution to journalArticle

Watanabe, Satoshi ; Matsumi, Rie ; Atomi, Haruyuki ; Imanaka, Tadayuki ; Miki, Kunio. / Crystal structures of the HypCD complex and the HypCDE ternary complex : Transient intermediate complexes during [NiFe] hydrogenase maturation. In: Structure. 2012 ; Vol. 20, No. 12. pp. 2124-2137.
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