Crystallization and preliminary crystallographic analysis of p40 phox, a regulatory subunit of NADPH oxidase

Kazuya Honbou, Satoru Yuzawa, Nobuo N. Suzuki, Yuko Fujioka, Hideki Sumimoto, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

p40phox is a cytosolic component of the phagocyte NADPH oxidase, which is responsible for production of the superoxide that kills invasive microorganisms. Full-length p40phox was expressed in Escherichia coli, purified and crystallized by the sitting-drop vapour-diffusion method at 293 K using polyethylene glycol 20 000 as a precipitant. Diffraction data were collected to 3.0 Å resolution at 100 K using synchrotron radiation. The crystal belongs to space group C2221, with unit-cell parameters a = 146.27, b = 189.81, c = 79.88 Å. This crystal was estimated to contain two or three protein molecules per asymmetric unit from the acceptable range of volume-to-weight ratio values.

Original languageEnglish
Pages (from-to)1018-1020
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number10
DOIs
Publication statusPublished - Oct 1 2006

Fingerprint

NADPH Oxidase
oxidase
Crystallization
crystallization
Crystals
Synchrotrons
inorganic peroxides
microorganisms
Phagocytes
Escherichia
Synchrotron radiation
Superoxides
Microorganisms
Escherichia coli
crystals
glycols
polyethylenes
synchrotron radiation
Diffraction
Vapors

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary crystallographic analysis of p40 phox, a regulatory subunit of NADPH oxidase. / Honbou, Kazuya; Yuzawa, Satoru; Suzuki, Nobuo N.; Fujioka, Yuko; Sumimoto, Hideki; Inagaki, Fuyuhiko.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 10, 01.10.2006, p. 1018-1020.

Research output: Contribution to journalArticle

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