Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli

Kaori Sasaki, Toyoyuki Ose, Taku Tanaka, Toshimi Mizukoshi, Tomoko Ishigaki, Katsumi Maenaka, Hisao Masai, Daisuke Kohda

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

PriA, a DEXH-type DNA helicase, binds specifically to the 3′ end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3′ terminus of DNA.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1764
Issue number1
DOIs
Publication statusPublished - Jan 1 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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